Salvati Diogo, Paschoalinotto Beatriz Helena, Mandim Filipa, Ferreira Isabel C F R, Steinmacher Nádia Cristiane, Pereira Carla, Dias Maria Inês
Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal.
Laboratório Associado Para a Sustentabilidade e Tecnologia em Regiões de Montanha (SusTEC), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal.
Foods. 2024 Feb 3;13(3):491. doi: 10.3390/foods13030491.
Germination is a natural, simple, and economical process used to improve the quality of nutritional and technological grains. In this study, native and sprouted sorghum flours were characterized regarding their technological properties (particle size distribution, water, and oil absorption capacity, swelling power and solubility, microscopy of starch granules, and pasting and thermal properties). Nutritional and phytochemical characterization profiles, including free sugars, fatty acids, organic acids, tocopherols, and phenolic compounds, were explored through chromatographic methods. The antioxidant, anti-inflammatory, and cytotoxic activities of the respective hydroethanolic extracts were also evaluated. The results showed that the germination process caused significant changes in the flour composition and properties, causing reduced gelatinization temperature and retarded starch retrogradation; an increased content of free sugars and total organic acids; and a decreased content of tocopherols and phenolic compounds. In terms of bioactivity, the sprouted sorghum flour extract showed better lipid-peroxidation-inhibition capacity and none of the extracts revealed hepatotoxicity or nephrotoxicity, which are important results for the validation of the use of the flours for food purposes. Germination is an efficient and alternative method for grain modification that gives improved technological properties without chemical modification or genetic engineering.
发芽是一种天然、简单且经济的过程,用于改善营养谷物和工艺谷物的品质。在本研究中,对天然高粱粉和发芽高粱粉的工艺特性(粒度分布、吸水性和吸油性、膨胀力和溶解性、淀粉颗粒显微镜观察以及糊化和热特性)进行了表征。通过色谱方法探究了营养和植物化学特性,包括游离糖、脂肪酸、有机酸、生育酚和酚类化合物。还评估了相应水乙醇提取物的抗氧化、抗炎和细胞毒性活性。结果表明,发芽过程导致面粉成分和特性发生显著变化,使糊化温度降低、淀粉回生延缓;游离糖和总有机酸含量增加;生育酚和酚类化合物含量降低。在生物活性方面,发芽高粱粉提取物表现出更好的脂质过氧化抑制能力,且所有提取物均未显示出肝毒性或肾毒性,这些都是验证面粉用于食品目的的重要结果。发芽是一种高效的谷物改良替代方法,无需化学改性或基因工程即可改善工艺特性。