Departamento de Ciencias de la Salud, Centro Universitario de los Valles, Universidad de Guadalajara, Carretera Guadalajara-Ameca Km. 45.5, Guadalajara 46600, Mexico.
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, A.P. 70228, Mexico City 04510, Mexico.
Int J Mol Sci. 2024 Jan 25;25(3):1460. doi: 10.3390/ijms25031460.
Some parasites are known to influence brain proteins or induce changes in the functioning of the nervous system. In this study, our objective is to demonstrate how the two-dimensional gel technique is valuable for detecting differences in protein expression and providing detailed information on changes in the brain proteome during a parasitic infection. Subsequently, we seek to understand how the parasitic infection affects the protein composition in the brain and how this may be related to changes in brain function. By analyzing de novo-expressed proteins at 2, 4, and 8 weeks post-infection compared to the brains of the control mice, we observed that proteins expressed at 2 weeks are primarily associated with neuroprotection or the initial response of the mouse brain to the infection. At 8 weeks, parasitic infection can induce oxidative stress in the brain, potentially activating signaling pathways related to the response to cellular damage. Proteins expressed at 8 weeks exhibit a pattern indicating that, as the host fails to balance the Neuro-Immuno-Endocrine network of the organism, the brain begins to undergo an apoptotic process and consequently experiences brain damage.
一些寄生虫已知会影响大脑蛋白质或诱导神经系统功能的变化。在这项研究中，我们的目的是展示二维凝胶技术如何有助于检测蛋白质表达的差异，并提供有关寄生虫感染过程中大脑蛋白质组变化的详细信息。随后，我们试图了解寄生虫感染如何影响大脑中的蛋白质组成，以及这可能与大脑功能的变化有何关联。通过分析与对照组小鼠相比，感染后 2、4 和 8 周新表达的蛋白质，我们观察到在 2 周时表达的蛋白质主要与神经保护或小鼠大脑对感染的初始反应有关。在 8 周时，寄生虫感染会导致大脑中的氧化应激，可能会激活与细胞损伤反应相关的信号通路。在 8 周时表达的蛋白质表现出一种模式，表明随着宿主无法平衡机体的神经-免疫-内分泌网络，大脑开始经历凋亡过程，进而导致脑损伤。
