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脂肪组织来源的干细胞分泌组在大鼠和小鼠完全性脊髓损伤后可诱导运动和组织学改善。

Adipose tissue derived stem cell secretome induces motor and histological gains after complete spinal cord injury in and mice.

作者信息

Assunção-Silva Rita C, Pinho Andreia, Cibrão Jorge R, Pereira Inês M, Monteiro Susana, Silva Nuno A, Campos Jonas, Rebelo Ana L, Schlosser Gerhard, Pinto Luisa, Pandit Abhay, Salgado António J

机构信息

Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.

ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.

出版信息

J Tissue Eng. 2024 Feb 9;15:20417314231203824. doi: 10.1177/20417314231203824. eCollection 2024 Jan-Dec.

DOI:10.1177/20417314231203824
PMID:38343771
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10858666/
Abstract

Mesenchymal stem cell-based therapies have been studied for spinal cord injury (SCI) treatment due to their paracrine action upon damaged tissues. MSCs neuroregenerative role may relate to the contents of their secretome in anti-inflammatory cytokines and growth-permissive factors. We propose using the secretome of MSCs isolated from the adipose tissue-adipose tissue-derived stem cells (ASCs) as a cell-free based therapy for SCI. In vivo studies were conducted in two SCI models, and mice, after complete spinal cord transection. Our results on both models demonstrated positive impacts of ASC secretome on their functional recovery which were correlated with histopathological markers of regeneration. Furthermore, in our mice study, secretome induced white matter preservation together with modulation of the local and peripheral inflammatory response. Altogether, these results demonstrate the neuroregenerative and potential for inflammatory modulation of ASC secretome suggesting it as a good candidate for cell-free therapeutic strategies for SCI.

摘要

基于间充质干细胞的疗法因其对受损组织的旁分泌作用而被用于脊髓损伤（SCI）治疗的研究。间充质干细胞的神经再生作用可能与其分泌组中抗炎细胞因子和生长促进因子的含量有关。我们建议使用从脂肪组织中分离的间充质干细胞——脂肪组织来源干细胞（ASCs）的分泌组作为SCI的无细胞疗法。在两种SCI模型（大鼠和小鼠）中进行了完全脊髓横断后的体内研究。我们在两种模型上的结果都证明了ASC分泌组对其功能恢复有积极影响，这与再生的组织病理学标志物相关。此外，在我们的小鼠研究中，分泌组诱导了白质保存以及局部和外周炎症反应的调节。总之，这些结果证明了ASC分泌组的神经再生和炎症调节潜力，表明它是SCI无细胞治疗策略的良好候选者。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/10858666/b3fd39c68a54/10.1177_20417314231203824-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/10858666/a0201f39c140/10.1177_20417314231203824-img2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/10858666/75f75ccb846e/10.1177_20417314231203824-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/10858666/078584531e24/10.1177_20417314231203824-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/10858666/eb05c961640f/10.1177_20417314231203824-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/10858666/98482a363ae4/10.1177_20417314231203824-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/10858666/f4920e6c41ae/10.1177_20417314231203824-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/10858666/a906b0349dbf/10.1177_20417314231203824-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/10858666/b3fd39c68a54/10.1177_20417314231203824-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/10858666/a0201f39c140/10.1177_20417314231203824-img2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/10858666/75f75ccb846e/10.1177_20417314231203824-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/10858666/078584531e24/10.1177_20417314231203824-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/10858666/eb05c961640f/10.1177_20417314231203824-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/10858666/98482a363ae4/10.1177_20417314231203824-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/10858666/f4920e6c41ae/10.1177_20417314231203824-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/10858666/a906b0349dbf/10.1177_20417314231203824-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d0/10858666/b3fd39c68a54/10.1177_20417314231203824-fig7.jpg

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