免疫组织化学鉴定感染 MfPV3 的食蟹猴（Macaca fascicularis）子宫和宫颈中整合素 alfa 6 的特征。

Immunohistochemical characterization of integrin alfa 6 in uterus and cervix of the cynomolgus monkeys (Macaca fascicularis) with MfPV3 infection.

机构信息

Primate Research Center, IPB University, Bogor, Indonesia.

Primatology Graduate Study Program, IPB University, Bogor, Indonesia.

出版信息

J Med Primatol. 2024 Feb;53(1):e12690. doi: 10.1111/jmp.12690.

DOI:10.1111/jmp.12690

PMID:38345331

Abstract

BACKGROUND

Cervical cancer is an abnormal growth of cervical tissue epithelial cells due to persistent human papilloma virus (HPV) infection. Cynomolgus monkeys (Macaca fascicularis) can be naturally and spontaneously infected with M. fascicularis Papillomavirus Type 3 (MfPV3), a virus that is phylogenetically closely related to human oncogenic HPV (HPV-16 and HPV-34), and therefore a potentially beneficial for modeling HPV disease. This study aims to evaluate the expression of the integrin alpha 6 (ITGα6) receptor in cynomolgus monkeys spontaneously infected with MfPV3, which this receptor also found in human infected with HPV.

METHODS

The study was done on archived Formalin-fixed Paraffin-Embedded (FFPE) samples of uterine and cervix tissue of cynomolgus monkeys. Immunohistochemistry was also performed to quantify the expression levels of ITGα6.

RESULTS

The results showed 80% of the samples positive Cervical Intraepithelial Neoplasia (CIN) and increased expression of ITGα6 significantly in Positive-MfPV3 group than negative-MfPV3 group.

CONCLUSIONS

This indicated the potential of cynomolgus monkeys as a spontaneous oncogenesis model of PV infection type.

摘要

背景

宫颈癌是由于持续的人乳头瘤病毒（HPV）感染导致宫颈组织上皮细胞异常生长。食蟹猴（Macaca fascicularis）可自然并自发感染 M. fascicularis 乳头瘤病毒 3 型（MfPV3），该病毒在进化上与人类致癌 HPV（HPV-16 和 HPV-34）密切相关，因此可能有益于 HPV 疾病的建模。本研究旨在评估整合素 alpha 6（ITGα6）受体在自然感染 MfPV3 的食蟹猴中的表达，该受体也存在于感染 HPV 的人类中。