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不同填充剂对分批补料堆肥及微生物群落特征的影响。

Effect of different bulking agents on fed-batch composting and microbial community profile.

作者信息

Wang Fei, Wang Jingyao, He Yuheng, Yan Yixin, Fu Dafang, Rene Eldon R, Singh Rajendra Prasad

机构信息

School of Civil Engineering, Southeast University, Nanjing, 211189, China.

School of Civil Engineering, Southeast University, Nanjing, 211189, China.

出版信息

Environ Res. 2024 May 15;249:118449. doi: 10.1016/j.envres.2024.118449. Epub 2024 Feb 13.

DOI:10.1016/j.envres.2024.118449
PMID:38354880
Abstract

The current study focused on analyzing the effect of different types of bulking agents and other factors on fed-batch composting and the structure of microbial communities. The results indicated that the introduction of bulking agents to fed-batch composting significantly improved composting efficiency as well as compost product quality. In particular, using green waste as a bulking agent, the compost products would achieve good performance in the following indicators: moisture (3.16%), weight loss rate (85.26%), and C/N ratio (13.98). The significant difference in moisture of compost products (p < 0.05) was observed in different sizes of bulking agent (green waste), which was because the voids in green waste significantly affected the capacity of the water to permeate. Meanwhile, controlling the size of green waste at 3-6 mm, the following indicators would show great performance from the compost products: moisture (3.12%), organic matter content (63.93%), and electrical conductivity (EC) (5.37 mS/cm). According to 16S rRNA sequencing, the relative abundance (RA) of thermophilic microbes increased as reactor temperature rose in fed-batch composting, among which Firmicutes, Proteobacteria, Basidiomycota, and Rasamsonia were involved in cellulose and lignocellulose degradation.

摘要

当前的研究聚焦于分析不同类型的膨松剂及其他因素对分批补料堆肥和微生物群落结构的影响。结果表明,在分批补料堆肥中引入膨松剂显著提高了堆肥效率以及堆肥产品质量。特别是,使用绿色废弃物作为膨松剂,堆肥产品在以下指标方面表现良好:湿度(3.16%)、失重率(85.26%)和碳氮比(13.98)。在不同尺寸的膨松剂(绿色废弃物)中观察到堆肥产品湿度存在显著差异(p < 0.05),这是因为绿色废弃物中的孔隙显著影响了水的渗透能力。同时,将绿色废弃物的尺寸控制在3 - 6毫米时,堆肥产品在以下指标方面表现出色:湿度(3.12%)、有机质含量(63.93%)和电导率(EC)(5.37毫西门子/厘米)。根据16S rRNA测序,在分批补料堆肥中,嗜热微生物的相对丰度随着反应器温度的升高而增加,其中厚壁菌门、变形菌门、担子菌门和拉氏菌属参与了纤维素和木质纤维素的降解。

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