Kronvall G, Simmons A, Myhre E B, Jonsson S
Infect Immun. 1979 Jul;25(1):1-10. doi: 10.1128/iai.25.1.1-10.1979.
Five gram-positive bacterial strains were selected for absorption studies of human serum samples. Strain AR1 (group A, M-type 1) and G148 (group G), with strong immunoglobulin G (IgG) binding capacities, and strain AW43 (group A, M-type 60), binding both IgA1 and IgA2, were compared with Staphylococcus aureus Cowan I and with Staphylococcus epidermidis L603. Both AR1 and G148 were capable of completely absorbing out serum IgG. In contrast, S. aureus Cowan I left a fraction unabsorbed, as expected from its known lack of IgG3 binding. Strain AW43 absorbed out all serum IgA, using a 10-microliter bacterial pellet for 20 microliter of serum. Serum IgM levels were slightly reduced by S. aureus Cowan I absorption. On the basis of the experiments, a bacterial mixture was designed consisting of S. aureus Cowan I and group A streptococcus strains AR1 and AW43, with absorption characteristics suitable for use in discriminating between early IgM and late IgG and IgA immune responses in routine serological work. A new type of bacteria-mammalian protein binding was discovered. Human serum albumin was completely absorbed out by strain G148 and to a lesser extent by strain AR1 and AW43. S. aureus Cowan I and S. epidermidis were negative. The binding capacity of G148 for albumin equalled that of Cowan I for IgG. The binding pattern of albumin to the strains was different from those of IgG, IgA, IgM, fibrinogen, haptoglobin, or aggregated beta 2-microglobulin and therefore seems to represent another type of bacterial-mammalian interaction with a specific albumin receptor on the surface of streptococci.
选择了5株革兰氏阳性细菌菌株用于人血清样本的吸收研究。将具有强免疫球蛋白G（IgG）结合能力的AR1菌株（A组，M型1）和G148菌株（G组），以及同时结合IgA1和IgA2的AW43菌株（A组，M型60）与金黄色葡萄球菌考恩I型和表皮葡萄球菌L603进行比较。AR1和G148均能够完全吸收血清IgG。相比之下，正如已知的金黄色葡萄球菌考恩I型缺乏IgG3结合能力所预期的那样，它会留下一部分未被吸收的IgG。AW43菌株使用10微升细菌沉淀处理20微升血清，可吸收所有血清IgA。金黄色葡萄球菌考恩I型吸收后血清IgM水平略有降低。基于这些实验，设计了一种由金黄色葡萄球菌考恩I型以及A组链球菌菌株AR1和AW43组成的细菌混合物，其吸收特性适用于在常规血清学检测中区分早期IgM和晚期IgG及IgA免疫反应。发现了一种新型的细菌 - 哺乳动物蛋白结合。人血清白蛋白被G148菌株完全吸收，AR1和AW43菌株吸收程度较小。金黄色葡萄球菌考恩I型和表皮葡萄球菌呈阴性。G148对白蛋白的结合能力与考恩I型对IgG的结合能力相当。白蛋白与这些菌株的结合模式不同于IgG、IgA、IgM、纤维蛋白原、触珠蛋白或聚集的β2微球蛋白，因此似乎代表了链球菌表面一种与特定白蛋白受体相互作用的新型细菌 - 哺乳动物相互作用类型。
