膜脂质过氧化的酶法测定

Enzymatic determination of membrane lipid peroxidation.

作者信息

Maiorino M, Roveri A, Ursini F, Gregolin C

出版信息

J Free Radic Biol Med. 1985;1(3):203-7. doi: 10.1016/0748-5514(85)90119-9.

Abstract

The recently purified "phospholipid hydroperoxide glutathione peroxidase" has been used to measure the membrane hydroperoxides formed during lipid peroxidation that are not substrates for the "classical" glutathione peroxidase. A spectrophotometric test in the presence of glutathione, glutathione reductase and NADPH has been used. The peroxidized membranes were added directly to the reaction mixture and the reaction was started by the addition of the enzyme. Triton X-100 exerted a stimulatory effect. Phospholipid hydroperoxide glutathione peroxidase allows a rapid, sensitive, accurate and specific determination of membrane hydroperoxides, the most quantitative index of lipid peroxidation. Glutathione peroxidase can be used in the same test to measure other hydroperoxides such as the cumene hydroperoxide used to induce the peroxidation.

摘要

最近纯化的“磷脂氢过氧化物谷胱甘肽过氧化物酶”已被用于测量脂质过氧化过程中形成的膜氢过氧化物，这些膜氢过氧化物不是“经典”谷胱甘肽过氧化物酶的底物。已采用在谷胱甘肽、谷胱甘肽还原酶和NADPH存在下的分光光度法检测。将过氧化的膜直接加入反应混合物中，并通过添加酶来启动反应。吐温X-100具有刺激作用。磷脂氢过氧化物谷胱甘肽过氧化物酶能够快速、灵敏、准确且特异地测定膜氢过氧化物，膜氢过氧化物是脂质过氧化最具定量性的指标。谷胱甘肽过氧化物酶可用于同一检测中，以测量其他氢过氧化物，例如用于诱导过氧化反应的异丙苯氢过氧化物。