Metallurgy and Materials Science Research Institute, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence in Natural Products for Ageing and Chronic Diseases, Chulalongkorn University, Bangkok 10330, Thailand.
Institute of Nutrition, Mahidol University, Nakhon Pathom 73170, Thailand.
Int J Biol Macromol. 2024 Apr;263(Pt 1):130264. doi: 10.1016/j.ijbiomac.2024.130264. Epub 2024 Feb 16.
A response surface methodology based on the Box-Behnken design was employed to develop fucoxanthin (FX) delivery nanocarrier from alginate (ALG) and chitosan (CS). The FX-loaded ALG/CS nanoparticles (FX-ALG/CS-NPs) were fabricated using oil-in-water emulsification and ionic gelation. The optimal formulation consisted of an ALG:CS mass ratio of 0.015:1, 0.71 % w/v Tween™ 80, and 5 mg/mL FX concentrations. The resulting FX-ALG/CS-NPs had a size of 227 ± 23 nm, a zeta potential of 35.3 ± 1.7 mV, and an encapsulation efficiency of 81.2 ± 2.8 %. These nanoparticles exhibited enhanced stability under simulated environmental conditions and controlled FX release in simulated gastrointestinal fluids. Furthermore, FX-ALG/CS-NPs showed increased in vitro oral bioaccessibility, gastrointestinal stability, antioxidant activity, anti-inflammatory effect, and cytotoxicity against various cancer cells. The findings suggest that ALG/CS-NPs are effective nanocarriers for the delivery of FX in nutraceuticals, functional foods, and pharmaceuticals.
采用基于 Box-Behnken 设计的响应面法，从海藻酸钠 (ALG) 和壳聚糖 (CS) 中开发出岩藻黄素 (FX) 递药纳米载体。使用油包水乳化法和离子凝胶法制备负载 FX 的 ALG/CS 纳米颗粒 (FX-ALG/CS-NPs)。最佳配方由 ALG:CS 质量比为 0.015:1、0.71%w/v Tween™ 80 和 5mg/mL FX 浓度组成。所得 FX-ALG/CS-NPs 的粒径为 227±23nm，Zeta 电位为 35.3±1.7mV，包封效率为 81.2±2.8%。这些纳米颗粒在模拟环境条件下表现出增强的稳定性，并在模拟胃肠道液中控制 FX 释放。此外，FX-ALG/CS-NPs 显示出体外口服生物利用度、胃肠道稳定性、抗氧化活性、抗炎作用和对各种癌细胞的细胞毒性增加。研究结果表明，ALG/CS-NPs 是在营养保健品、功能性食品和药物中递送 FX 的有效纳米载体。
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