Australian National Phenome Centre, Health Futures Institute, Harry Perkins Institute, Murdoch University, 5 Robin Warren Drive, Murdoch, WA 6150, Australia.
Centre for Computational and Systems Medicine, Health Futures Institute, Harry Perkins Institute, Murdoch University, 5 Robin Warren Drive, Murdoch, WA 6150, Australia.
Anal Chem. 2024 Mar 19;96(11):4505-4513. doi: 10.1021/acs.analchem.3c05152. Epub 2024 Feb 19.
We investigated plasma and serum blood derivatives from capillary blood microsamples (500 μL, MiniCollect tubes) and corresponding venous blood (10 mL vacutainers). Samples from 20 healthy participants were analyzed by H NMR, and 112 lipoprotein subfraction parameters; 3 supramolecular phospholipid composite (SPC) parameters from SPC, SPC, and SPC subfractions; 2 -acetyl signals from α-1-acid glycoprotein (Glyc), GlycA, and GlycB; and 3 calculated parameters, SPC (total), SPC/SPC, and Glyc (total) were assessed. Using linear regression between capillary and venous collection sites, we explained that agreement (Adj. ≥ 0.8, < 0.001) was witnessed for 86% of plasma parameters (103/120) and 88% of serum parameters (106/120), indicating that capillary lipoprotein, SPC, and Glyc concentrations follow changes in venous concentrations. These results indicate that capillary blood microsamples are suitable for sampling in remote areas and for high-frequency longitudinal sampling of the majority of lipoproteins, SPCs, and Glycs.
我们研究了来自毛细血管血微样本（500μL，MiniCollect 管）和相应静脉血（10mL 真空采血管）的血浆和血清血液衍生物。对 20 名健康参与者的样本进行了 H NMR 分析和 112 个脂蛋白亚组分参数分析；来自 SPC、SPC 和 SPC 亚组分的 3 个超分子磷脂复合（SPC）参数；来自α-1-酸性糖蛋白（Glyc）、GlycA 和 GlycB 的 2 个 -乙酰信号；以及 3 个计算参数，SPC（总）、SPC/SPC 和 Glyc（总）。通过毛细管和静脉采集部位之间的线性回归，我们发现 86%的血浆参数（103/120）和 88%的血清参数（106/120）具有良好的一致性（Adj. ≥0.8，<0.001），表明毛细血管脂蛋白、SPC 和 Glyc 浓度随静脉浓度的变化而变化。这些结果表明，毛细血管血微样本适用于偏远地区的采样以及大多数脂蛋白、SPC 和 Glyc 的高频纵向采样。
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