一种用于检测人血浆和血清样本中SARS-CoV-2刺突特异性IgG的高效酶联免疫吸附测定方案。
An efficient ELISA protocol for measurement of SARS-CoV-2 spike-specific IgG in human plasma and serum samples.
作者信息
Appeltrath Gwenllian A, Parreuter Janine, Lindemann Monika, Klump Hannes, Karsten Christina B
机构信息
Institute for the Research on HIV and AIDS-associated Diseases, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
出版信息
MethodsX. 2024 Feb 5;12:102596. doi: 10.1016/j.mex.2024.102596. eCollection 2024 Jun.
Here, we describe a protocol for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike-specific immunoglobulin G (IgG) by enzyme-linked immunosorbent assay (ELISA). The protocol was developed with a keen focus on optimizing several key parameters, including antigen coating concentration, antibody and sample dilutions, and assay development time. The final protocol features the following characteristics:•The capability to detect SARS-CoV-2 spike-specific IgG in both plasma and serum samples.•A streamlined procedure that requires only 1 hour and 20 minutes of hands-on time.•Reliable assay performance, with a remarkable sensitivity of 98.1 % and specificity of 99.5 %.
在此，我们描述了一种通过酶联免疫吸附测定（ELISA）检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）刺突特异性免疫球蛋白G（IgG）的方法。该方法在开发过程中重点优化了几个关键参数，包括抗原包被浓度、抗体和样品稀释度以及检测开发时间。最终方法具有以下特点：
能够检测血浆和血清样本中的SARS-CoV-2刺突特异性IgG。
流程简化，实际操作时间仅需1小时20分钟。
检测性能可靠，灵敏度高达98.1%，特异性为99.5%。
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