Yan Yuxiao, Wang Conglian, Wan Renyuan, Li Shuang, Yang Yanfen, Lv Caiyou, Li Yongmei, Yang Guangrong
College of Tea Science, Yunnan Agricultural University, Kunming, Yunnan, China.
College of Resources and Environment, Yunnan Agricultural University, Kunming, Yunnan, China.
Front Microbiol. 2024 Feb 7;15:1334711. doi: 10.3389/fmicb.2024.1334711. eCollection 2024.
Polyethylene mulch is a kind of inorganic mulch widely used in agriculture. The effects of plastic mulch debris on the structure of plant soil and root growth have been fully studied, but their effects on endophytic microbial communities have not been explored to a large extent.
In this study, High-throughput sequencing of bacterial 16S rRNA genes and fungal ITS region sequences were used to analyze microbial community structure and composition in rhizosphere soil and root endophytic of tea plant under three different weeding methods: polyethylene mulching, hand weeding and no weeding (CK).
The results showed that the weeding methods had no significant effect on the rhizosphere and root endophytic microbial abundance, but the rhizosphere bacterial structure covered by polyethylene mulch was significantly different than hand weeding and CK. The rhizosphere fungal diversity was also significantly higher than the other two analyzed treatments. The community abundance of rhizosphere microorganisms Acidobacteria, Candidatus Rokubacteria and covered by polyethylene mulch decreased significantly, whereas , and increased significantly. The abundance of bacteria , , and communities covered by polyethylene mulch was significantly changed, and the abundance of and Nitrobacteraceae was significantly increased. Rhizosphere dominant bacteria were negatively correlated with soil available nitrogen content, while dominant fungi were significantly correlated with soil pH, total nitrogen and total potassium.
Polyethylene mulch forms an independent micro-ecological environment. At the same time, the soil nutrient environment was enriched by affecting the nitrogen cycle, and the composition of microbial community was affected. This study elucidated the effects of polyethylene mulch on soil microbial community in tea garden and provided a new theoretical understanding for weed management.
聚乙烯地膜是一种广泛应用于农业的无机地膜。塑料地膜碎片对植物土壤结构和根系生长的影响已得到充分研究，但其对内生微生物群落的影响在很大程度上尚未被探索。
本研究采用细菌16S rRNA基因和真菌ITS区域序列的高通量测序，分析了三种不同除草方法下茶树根际土壤和根内生微生物群落结构及组成：聚乙烯地膜覆盖、人工除草和不除草（对照）。
结果表明，除草方法对根际和根内生微生物丰度无显著影响，但聚乙烯地膜覆盖下的根际细菌结构与人工除草和对照有显著差异。根际真菌多样性也显著高于其他两种分析处理。聚乙烯地膜覆盖下的根际微生物酸杆菌门、暂定罗库菌门的群落丰度显著降低，而芽单胞菌门、浮霉菌门和绿弯菌门显著增加。聚乙烯地膜覆盖下的细菌伯克氏菌属、慢生根瘤菌属、甲基杆菌属和硝化螺旋菌属群落丰度发生显著变化，硝化弧菌属和硝化杆菌科的丰度显著增加。根际优势细菌与土壤有效氮含量呈负相关，而优势真菌与土壤pH值、全氮和全钾显著相关。
聚乙烯地膜形成了一个独立的微生态环境。同时，通过影响氮循环丰富了土壤养分环境，影响了微生物群落组成。本研究阐明了聚乙烯地膜对茶园土壤微生物群落的影响，为杂草管理提供了新的理论认识。
