基于 CRISPR/Cas13a 的生物传感器在血清标志物检测中的应用。
Application of CRISPR/Cas13a-based biosensors in serum marker detection.
机构信息
State Key Laboratory of Targeting Oncology, National Center for International Research of Bio-Targeting Theranostics, Guangxi Key Laboratory of Bio-Targeting Theranostics, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning 530021, China.
College of Chemistry, Guangdong University of Petrochemical Technology, Guandu Road, Maoming, Guangdong 525000, China.
出版信息
Anal Methods. 2024 Mar 7;16(10):1426-1438. doi: 10.1039/d3ay01927f.
The detection of serum markers is important for the early diagnosis and monitoring of diseases, but conventional detection methods have the problem of low specificity or sensitivity. CRISPR/Cas13a-based biosensors have the characteristics of simple detection methods and high sensitivity, which have a certain potential to solve the problems of conventional detection. This paper focuses on the research progress of CRISPR/Cas13a-based biosensors in serum marker detection, introduces the principles and applications of fluorescence, electrochemistry, colorimetric, and other biosensors based on CRISPR/Cas13a in the detection of serum markers, compares and analyzes the differences between the above CRISPR/Cas13a-based biosensors, and looks forward to the future development direction of CRISPR/Cas13a-based biosensors.
血清标志物的检测对于疾病的早期诊断和监测很重要，但传统的检测方法存在特异性或灵敏度低的问题。基于 CRISPR/Cas13a 的生物传感器具有检测方法简单、灵敏度高的特点，在一定程度上有解决传统检测问题的潜力。本文重点介绍了基于 CRISPR/Cas13a 的生物传感器在血清标志物检测中的研究进展，介绍了基于 CRISPR/Cas13a 的荧光、电化学、比色等生物传感器在血清标志物检测中的原理和应用，比较分析了上述基于 CRISPR/Cas13a 的生物传感器的差异，并对基于 CRISPR/Cas13a 的生物传感器的未来发展方向进行了展望。
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