Liu Yang, An Jiahao, Safdar Asma, Shen Yang, Sun Yang, Shu Wenhui, Tan Xiaojuan, Zhu Bo, Xiao Jiaxin, Schirawski Jan, He Feng, Zhu Guoping
College of Life Sciences, Anhui Normal University, Wuhu 241000, China.
Department of Plant Pathology, College of Agriculture, University of Sargodha, Sargodha 40100, Pakistan.
J Fungi (Basel). 2024 Feb 16;10(2):156. doi: 10.3390/jof10020156.
Rice production in the Anhui province is threatened by fungal diseases. We obtained twenty-five fungal isolates from rice and wild rice leaves showing leaf spot disease collected along the Yangtze River. A phylogenetic analysis based on internal transcribed spacer (ITS), translation elongation factor 1 alpha (), and beta tubulin () sequences revealed one isolate (SS-2-JB-1B) grouped with , one (QY) with , twenty-two with , and one isolate (QY-2) grouped in its own clade, which are related to but clearly different from . Nineteen tested isolates, including sixteen strains from the clade and the three isolates of the other three clades, caused disease on detached rice leaves. The three isolates that did not belong to were also able to cause disease in rice seedlings, suggesting that they were rice pathogens. Isolate QY-2 differed from the other isolates in terms of colony morphology, cell size, and susceptibility to fungicides, indicating that this isolate represents a new species that we named . Our analysis showed that , , and the new species, , can cause rice leaf spot disease in the field. This research provides new knowledge for understanding rice leaf spot disease.
安徽省的水稻生产受到真菌病害的威胁。我们从沿长江采集的表现出叶斑病的水稻和野生稻叶片中获得了25个真菌分离株。基于内转录间隔区（ITS）、翻译延伸因子1α（）和β微管蛋白（）序列的系统发育分析表明，一个分离株（SS-2-JB-1B）与聚为一类，一个（QY）与聚为一类，22个与聚为一类，还有一个分离株（QY-2）聚在其自己的分支中，它们与相关但明显不同。19个测试分离株，包括来自分支的16个菌株和其他三个分支的3个分离株，在离体水稻叶片上引起了病害。不属于的这3个分离株也能够在水稻幼苗上引起病害，表明它们是水稻病原菌。分离株QY-2在菌落形态、细胞大小和对杀菌剂的敏感性方面与其他分离株不同，表明该分离株代表一个我们命名为的新物种。我们的分析表明，、和这个新物种能够在田间引起水稻叶斑病。这项研究为理解水稻叶斑病提供了新知识。
