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冷应激条件下鸡胚细胞中积累的无活性核糖体复合物的低温电镜结构。

Cryo-EM structure of the inactive ribosome complex accumulated in chick embryo cells in cold-stress conditions.

机构信息

Integrated Structural Biology, IGBMC - IGBMC - CNRS UMR 7104, Inserm, France.

Dubochet Center for Imaging (DCI), EPFL, Lausanne, Switzerland.

出版信息

FEBS Lett. 2024 Mar;598(5):537-547. doi: 10.1002/1873-3468.14831. Epub 2024 Feb 23.

Abstract

Here, we present the high-resolution structure of the Gallus gallus 80S ribosome obtained from cold-treated chicken embryos. The translationally inactive ribosome complex contains elongation factor eEF2 with GDP, SERPINE1 mRNA binding protein 1 (SERBP1) and deacylated tRNA in the P/E position, showing common features with complexes already described in mammals. Modeling of most expansion segments of G. gallus 28S ribosomal RNA allowed us to identify specific features in their structural organization and to describe areas where a marked difference between mammalian and avian ribosomes could shed light on the evolution of the expansion segments. This study provides the first structure of an avian ribosome, establishing a model for future structural and functional studies on the translational machinery in Aves.

摘要

在这里,我们展示了来自冷处理鸡胚的 Gallus gallus 80S 核糖体的高分辨率结构。该翻译无活性的核糖体复合物包含 GDP 结合的延伸因子 eEF2、SERPINE1 mRNA 结合蛋白 1(SERBP1)和去酰化的 tRNA 在 P/E 位,显示出与已在哺乳动物中描述的复合物的共同特征。对 G. 鸡 28S 核糖体 RNA 的大多数扩展片段进行建模,使我们能够识别其结构组织中的特定特征,并描述在哺乳动物和禽类核糖体之间存在明显差异的区域,这可以阐明扩展片段的进化。本研究提供了第一个禽类核糖体的结构,为未来在鸟类中转录机器的结构和功能研究建立了模型。

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