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120 微特斯拉下的极化丙酮酸的 C MRI。

C MRI of hyperpolarized pyruvate at 120 µT.

机构信息

High-Field Magnetic Resonance Center, Max Planck Institute for Biological Cybernetics, 72076, Tübingen, Germany.

Physikalisch-Technische Bundesanstalt, 10587, Berlin, Germany.

出版信息

Sci Rep. 2024 Feb 23;14(1):4468. doi: 10.1038/s41598-024-54770-x.


DOI:10.1038/s41598-024-54770-x
PMID:38396023
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10891046/
Abstract

Nuclear spin hyperpolarization increases the sensitivity of magnetic resonance dramatically, enabling many new applications, including real-time metabolic imaging. Parahydrogen-based signal amplification by reversible exchange (SABRE) was employed to hyperpolarize [1-C]pyruvate and demonstrate C imaging in situ at 120 µT, about twice Earth's magnetic field, with two different signal amplification by reversible exchange variants: SABRE in shield enables alignment transfer to heteronuclei (SABRE-SHEATH), where hyperpolarization is transferred from parahydrogen to [1-C]pyruvate at a magnetic field below 1 µT, and low-irradiation generates high tesla (LIGHT-SABRE), where hyperpolarization was prepared at 120 µT, avoiding magnetic field cycling. The 3-dimensional images of a phantom were obtained using a superconducting quantum interference device (SQUID) based magnetic field detector with submillimeter resolution. These C images demonstrate the feasibility of low-field C metabolic magnetic resonance imaging (MRI) of 50 mM [1-C]pyruvate hyperpolarized by parahydrogen in reversible exchange imaged at about twice Earth's magnetic field. Using thermal C polarization available at 120 µT, the same experiment would have taken about 300 billion years.

摘要

核自旋超极化极大地提高了磁共振的灵敏度,使许多新的应用成为可能,包括实时代谢成像。采用基于 Para 氢气的可逆交换信号放大(SABRE)对 [1-C]丙酮酸进行超极化,并在约为地球磁场两倍的 120 µT 磁场下原位演示 C 成像,使用了两种不同的可逆交换变体:屏蔽中的 SABRE 可实现核间对准转移(SABRE-SHEATH),其中在低于 1 µT 的磁场下将超极化从 Para 氢气转移到 [1-C]丙酮酸,而低辐照产生高特斯拉(LIGHT-SABRE),其中在 120 µT 下制备超极化,避免磁场循环。使用具有亚毫米分辨率的超导量子干涉装置(SQUID)磁场探测器获得了幻影的 3D 图像。这些 C 图像证明了在约为地球磁场两倍的磁场下,使用可逆交换对 50 mM [1-C]丙酮酸进行 Para 氢气超极化,并进行 C 代谢磁共振成像(MRI)的可行性。利用在 120 µT 时可获得的热 C 极化,相同的实验将需要大约 3000 亿年。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903e/10891046/767ceb43c982/41598_2024_54770_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903e/10891046/25e8651d7d6c/41598_2024_54770_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903e/10891046/b5db471dda51/41598_2024_54770_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903e/10891046/767ceb43c982/41598_2024_54770_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903e/10891046/25e8651d7d6c/41598_2024_54770_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903e/10891046/b5db471dda51/41598_2024_54770_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903e/10891046/767ceb43c982/41598_2024_54770_Fig3_HTML.jpg

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C MRI of hyperpolarized pyruvate at 120 µT.

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[3]
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[6]
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[7]
LIGHT-SABRE Hyperpolarizes 1-C-Pyruvate Continuously without Magnetic Field Cycling.

J Phys Chem C Nanomater Interfaces. 2023-4-4

[8]
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[9]
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[10]
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引用本文的文献

[1]
Indirect Zero-Field Nuclear Magnetic Resonance Spectroscopy.

Anal Chem. 2025-8-19

本文引用的文献

[1]
Facile hyperpolarization chemistry for molecular imaging and metabolic tracking of [1-C]pyruvate in vivo.

J Magn Reson Open. 2023-12

[2]
In Vivo Metabolic Imaging of [1- C]Pyruvate-d Hyperpolarized By Reversible Exchange With Parahydrogen.

Angew Chem Int Ed Engl. 2023-9-4

[3]
Over 20% Carbon-13 Polarization of Perdeuterated Pyruvate Using Reversible Exchange with Parahydrogen and Spin-Lock Induced Crossing at 50 μT.

J Phys Chem Lett. 2023-6-15

[4]
LIGHT-SABRE Hyperpolarizes 1-C-Pyruvate Continuously without Magnetic Field Cycling.

J Phys Chem C Nanomater Interfaces. 2023-4-4

[5]
Catalyst-Free Aqueous Hyperpolarized [1-C]Pyruvate Obtained by Re-Dissolution Signal Amplification by Reversible Exchange.

ACS Sens. 2022-11-25

[6]
RASER MRI: Magnetic resonance images formed spontaneously exploiting cooperative nonlinear interaction.

Sci Adv. 2022-7-15

[7]
Recent advances in the application of parahydrogen in catalysis and biochemistry.

RSC Adv. 2022-4-26

[8]
Temperature Cycling Enables Efficient C SABRE-SHEATH Hyperpolarization and Imaging of [1-C]-Pyruvate.

J Am Chem Soc. 2022-1-12

[9]
Order-Unity C Nuclear Polarization of [1- C]Pyruvate in Seconds and the Interplay of Water and SABRE Enhancement.

Chemphyschem. 2022-1-19

[10]
Optimisation of pyruvate hyperpolarisation using SABRE by tuning the active magnetisation transfer catalyst.

Catal Sci Technol. 2020-3-7

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