High-Field Magnetic Resonance Center, Max Planck Institute for Biological Cybernetics, 72076, Tübingen, Germany.
Physikalisch-Technische Bundesanstalt, 10587, Berlin, Germany.
Sci Rep. 2024 Feb 23;14(1):4468. doi: 10.1038/s41598-024-54770-x.
Nuclear spin hyperpolarization increases the sensitivity of magnetic resonance dramatically, enabling many new applications, including real-time metabolic imaging. Parahydrogen-based signal amplification by reversible exchange (SABRE) was employed to hyperpolarize [1-C]pyruvate and demonstrate C imaging in situ at 120 µT, about twice Earth's magnetic field, with two different signal amplification by reversible exchange variants: SABRE in shield enables alignment transfer to heteronuclei (SABRE-SHEATH), where hyperpolarization is transferred from parahydrogen to [1-C]pyruvate at a magnetic field below 1 µT, and low-irradiation generates high tesla (LIGHT-SABRE), where hyperpolarization was prepared at 120 µT, avoiding magnetic field cycling. The 3-dimensional images of a phantom were obtained using a superconducting quantum interference device (SQUID) based magnetic field detector with submillimeter resolution. These C images demonstrate the feasibility of low-field C metabolic magnetic resonance imaging (MRI) of 50 mM [1-C]pyruvate hyperpolarized by parahydrogen in reversible exchange imaged at about twice Earth's magnetic field. Using thermal C polarization available at 120 µT, the same experiment would have taken about 300 billion years.
核自旋超极化极大地提高了磁共振的灵敏度,使许多新的应用成为可能,包括实时代谢成像。采用基于 Para 氢气的可逆交换信号放大(SABRE)对 [1-C]丙酮酸进行超极化,并在约为地球磁场两倍的 120 µT 磁场下原位演示 C 成像,使用了两种不同的可逆交换变体:屏蔽中的 SABRE 可实现核间对准转移(SABRE-SHEATH),其中在低于 1 µT 的磁场下将超极化从 Para 氢气转移到 [1-C]丙酮酸,而低辐照产生高特斯拉(LIGHT-SABRE),其中在 120 µT 下制备超极化,避免磁场循环。使用具有亚毫米分辨率的超导量子干涉装置(SQUID)磁场探测器获得了幻影的 3D 图像。这些 C 图像证明了在约为地球磁场两倍的磁场下,使用可逆交换对 50 mM [1-C]丙酮酸进行 Para 氢气超极化,并进行 C 代谢磁共振成像(MRI)的可行性。利用在 120 µT 时可获得的热 C 极化,相同的实验将需要大约 3000 亿年。
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