Nakamura H, Tonosaki A, Washioka H, Takahashi K, Yasui S
Cell Tissue Res. 1985;241(3):523-8. doi: 10.1007/BF00214571.
Perfusion fixation via pulmonary trunk was applied to the alveolar lining layer in situ at different lung volumes using a fixative containing tannic acid-ferrocyanide osmium. The monomolecular surface film and hypophasic tubular myelin figures were enhanced. In the range of transpulmonary pressure (1-10 cmH2O), the surface film appeared in the form of a single, electron-dense leaflet, 2.7 +/- 0.6 nm (M +/- SD) in thickness while trilaminar membrane structure was retained in all parts of the tubular myelin figures of the hypophase. The surface film was attached underneath at right angles with trilaminar membranes which formed the outermost parts of the tubular myelin. Such structural continuity was taken to support a view that the phospholipid unit membrane of the tubular myelin figure would be transformed at the hydrophobic phase into a pair of monomolecular leaflets, eventually forming the surface film.
通过肺动脉进行灌注固定，在不同肺容积下对肺泡内衬层进行原位固定，使用含有鞣酸 - 亚铁氰化锇的固定剂。单分子表面膜和亚相管状髓鞘样结构得到增强。在跨肺压（1 - 10 cmH₂O）范围内，表面膜呈现为单一的、电子致密小叶形式，厚度为2.7 +/- 0.6 nm（平均值 +/- 标准差），而亚相的管状髓鞘样结构的所有部分均保留三层膜结构。表面膜以直角附着在形成管状髓鞘最外层部分的三层膜下方。这种结构连续性支持了一种观点，即管状髓鞘样结构的磷脂单位膜在疏水相将转化为一对单分子小叶，最终形成表面膜。
