Van Damme M, Prevost M
Comput Programs Biomed. 1985;19(2-3):107-17. doi: 10.1016/0010-468x(85)90001-7.
The glomerular capillary wall of the kidney behaves as an electronegatively charged structure consisting of three layers, the lamina densa and the two laminae rarae, which are differently charged. Thus, a three layer model is proposed to analyse the transport of charged macromolecules across this wall. A modified Nernst-Planck equation describes the macromolecule flux across the wall and a Donnan equilibrium is assumed at each interface. For a given value of the fixed charge concentration in each layer, the local sieving coefficient of the macromolecule, i.e. the ratio between the concentrations in the filtrate and in the plasma, is calculated. A sieving curve which relates the sieving coefficient to the Einstein-Stokes radius of the macrosolute is obtained. The fixed charge concentrations in each layer are iteratively modified until simultaneous adjustment is achieved between calculated and experimental curves, for positively and negatively charged tracers and their neutral equivalent.
肾脏的肾小球毛细血管壁表现为一个带负电荷的结构，由三层组成，即致密层和两层疏松层，它们带有不同的电荷。因此，提出了一个三层模型来分析带电大分子穿过该壁的运输。一个修正的能斯特 - 普朗克方程描述了大分子穿过壁的通量，并假设在每个界面处存在唐南平衡。对于每层中固定电荷浓度的给定值，计算大分子的局部筛分系数，即滤液和血浆中浓度的比值。得到了一条将筛分系数与大分子溶质的爱因斯坦 - 斯托克斯半径相关联的筛分曲线。反复修改每层中的固定电荷浓度，直到对于带正电和带负电的示踪剂及其中性等效物，计算曲线和实验曲线之间实现同时调整。
