Dowaidar Moataz
Bioengineering Department, King Fahd University of Petroleum and Minerals (KFUPM), Dhahran 31261, Saudi Arabia; Interdisciplinary Research Center for Hydrogen Technologies and Carbon Management, King Fahd University of Petroleum and Minerals (KFUPM), Dhahran 31261, Saudi Arabia; Biosystems and Machines Research Center, King Fahd University of Petroleum and Minerals (KFUPM), Dhahran 31261, Saudi Arabia.
Cell Signal. 2024 May;117:111116. doi: 10.1016/j.cellsig.2024.111116. Epub 2024 Feb 24.
Cell-penetrating peptides have been extensively utilized for the purpose of facilitating the intracellular delivery of cargo that is impermeable to the cell membrane. The researchers have exhibited proficient delivery capabilities for oligonucleotides, thereby establishing cell-penetrating peptides as a potent instrument in the field of gene therapy. Furthermore, they have demonstrated a high level of efficiency in delivering several additional payloads. Cell penetrating peptides (CPPs) possess the capability to efficiently transport therapeutic molecules to specific cells, hence offering potential remedies for many illnesses. Hence, their utilization is imperative for the improvement of therapeutic vaccines. In contemporary studies, a plethora of cell-penetrating peptides have been unveiled, each characterized by its own distinct structural attributes and associated mechanisms. Although it is widely acknowledged that there are multiple pathways through which particles might be internalized, a comprehensive understanding of the specific mechanisms by which these particles enter cells has to be fully elucidated. The absorption of cell-penetrating peptides can occur through either direct translocation or endocytosis. However, it is worth noting that categories of cell-penetrating peptides are not commonly linked to specific entrance mechanisms. Furthermore, research has demonstrated that cell-penetrating peptides (CPPs) possess the capacity to enhance antigen uptake by cells and facilitate the traversal of various biological barriers. The primary objective of this work is to examine the mechanisms by which cell-penetrating peptides are internalized by cells and their significance in facilitating the administration of drugs, particularly in the context of gene therapy and vaccine development. The current study investigates the immunostimulatory properties of numerous vaccine components administered using different cell-penetrating peptides (CPPs). This study encompassed a comprehensive discussion on various topics, including the uptake pathways and mechanisms of cell-penetrating peptides (CPPs), the utilization of CPPs as innovative vectors for gene therapy, the role of CPPs in vaccine development, and the potential of CPPs for antigen delivery in the context of vaccine development.
细胞穿透肽已被广泛用于促进细胞膜不可渗透的货物的细胞内递送。研究人员已展示出对寡核苷酸的高效递送能力，从而将细胞穿透肽确立为基因治疗领域的一种有效工具。此外，他们在递送多种其他载荷方面也表现出了很高的效率。细胞穿透肽（CPPs）能够有效地将治疗分子运输到特定细胞，因此为许多疾病提供了潜在的治疗方法。因此，它们的使用对于改进治疗性疫苗至关重要。在当代研究中，已经发现了大量的细胞穿透肽，每种都具有其独特的结构特征和相关机制。尽管人们普遍认为颗粒可能通过多种途径被内化，但必须充分阐明这些颗粒进入细胞的具体机制。细胞穿透肽的吸收可以通过直接转位或内吞作用发生。然而，值得注意的是，细胞穿透肽的类别通常与特定的进入机制无关。此外，研究表明细胞穿透肽（CPPs）具有增强细胞对抗原的摄取并促进穿越各种生物屏障的能力。这项工作的主要目的是研究细胞穿透肽被细胞内化的机制及其在促进药物给药方面的意义，特别是在基因治疗和疫苗开发的背景下。当前的研究调查了使用不同细胞穿透肽（CPPs）给药的多种疫苗成分的免疫刺激特性。这项研究全面讨论了各种主题，包括细胞穿透肽（CPPs）的摄取途径和机制、CPPs作为基因治疗的创新载体的应用、CPPs在疫苗开发中的作用以及CPPs在疫苗开发背景下用于抗原递送的潜力。
