Lopez Maury Luis, Ren Liping, Hassan Shaimaa, Bähler Jürg, Gould Kathleen L
Department of Genetics, Evolution, and Environment, Institute of Healthy Ageing, University College London, London, UK.
Current: Instituto de Bioquimica Vegetal y Fotosintesis, Universidad de Sevilla, Sevilla, Spain.
MicroPubl Biol. 2024 Feb 12;2024. doi: 10.17912/micropub.biology.001089. eCollection 2024.
Clp1 is a Cdc14-family phosphatase that reverses mitotic Cdk1 phosphorylation. Despite evolutionary conservation, Clp1 's mammalian orthologs do not share this function. Rather, higher eukaryotic Cdc14 enzymes act in DNA repair, ciliogenesis, and gene regulation. To examine if Clp1 regulates gene expression, we compared the transcriptional profiles of cells lacking Clp1 function to that of wildtype. Because cells are sensitive to the actin depolymerizing drug, LatrunculinA, we also investigated whether a transcriptional response was involved. Our results indicate that Clp1 does not detectably affect gene expression and highlight the organism-specific functions of this conserved phosphatase family.
Clp1是一种Cdc14家族磷酸酶，可逆转有丝分裂Cdk1磷酸化。尽管具有进化保守性，但Clp1的哺乳动物直系同源物并不具备此功能。相反，高等真核生物的Cdc14酶在DNA修复、纤毛发生和基因调控中发挥作用。为了研究Clp1是否调控基因表达，我们将缺乏Clp1功能的细胞与野生型细胞的转录谱进行了比较。由于细胞对肌动蛋白解聚药物LatrunculinA敏感，我们还研究了是否涉及转录反应。我们的结果表明，Clp1对基因表达没有可检测到的影响，并突出了这个保守磷酸酶家族的物种特异性功能。
