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建立膀胱癌球体并在微流控平台中培养以预测药物反应。

Establishment of bladder cancer spheroids and cultured in microfluidic platform for predicting drug response.

作者信息

Xiong Qiao, Liu Ting, Ying Yidie, Yu Xiaowen, Wang Ziwei, Gao Hongliang, Lin Tianhai, Fan Weihua, Zhang Zhensheng, Wei Qiang, Ge Yuqing, Zeng Shuxiong, Xu Chuanliang

机构信息

Department of Urology Institute of Urology, West China Hospital, Sichuan University Chengdu P. R. China.

Department of Urology Changhai Hospital, Naval Medical University Shanghai P. R. China.

出版信息

Bioeng Transl Med. 2023 Dec 4;9(2):e10624. doi: 10.1002/btm2.10624. eCollection 2024 Mar.

DOI:10.1002/btm2.10624
PMID:38435820
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10905551/
Abstract

Cisplatin-containing combination chemotherapy has been used as the standard treatment for bladder cancer patients at advanced stage. However, nearly 50% of patients are nonresponders. To guide the selection of more effective chemotherapeutic agents, a bladder cancer spheroids microfluidic drug sensitivity analysis system was established in this study. Bladder cancer spheroids were established and successfully cultured in a customized microfluidic device to assess their response to different chemotherapeutic agents. The in vitro drug sensitivity results were also compared to patient-derived xenograft (PDX) models and clinical responses of patients. As a result, bladder cancer spheroids faithfully recapitulate the histopathological and genetic features of their corresponding parental tumors. Furthermore, the in vitro drug sensitivity outcomes of spheroids ( = 8) demonstrated a high level of correlation with the PDX ( = 2) and clinical response in patients ( = 2). Our study highlights the potential of combining bladder cancer spheroids and microfluidic devices as an efficient and accurate platform for personalized selection of chemotherapeutic agents.

摘要

含顺铂的联合化疗一直被用作晚期膀胱癌患者的标准治疗方法。然而，近50%的患者没有反应。为了指导选择更有效的化疗药物，本研究建立了一种膀胱癌球体微流控药物敏感性分析系统。在定制的微流控装置中建立并成功培养了膀胱癌球体，以评估它们对不同化疗药物的反应。体外药物敏感性结果还与患者来源的异种移植（PDX）模型以及患者的临床反应进行了比较。结果，膀胱癌球体忠实地再现了其相应亲本肿瘤的组织病理学和遗传特征。此外，球体（n = 8）的体外药物敏感性结果与PDX（n = 2）和患者的临床反应（n = 2）显示出高度相关性。我们的研究突出了将膀胱癌球体和微流控装置相结合作为个性化选择化疗药物的高效且准确平台的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0771/10905551/007d2a5a35e1/BTM2-9-e10624-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0771/10905551/ae59a73f7960/BTM2-9-e10624-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0771/10905551/5b9270fcb5d1/BTM2-9-e10624-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0771/10905551/4e32a57b50fa/BTM2-9-e10624-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0771/10905551/c7a63739544d/BTM2-9-e10624-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0771/10905551/a5ac7e02d8e3/BTM2-9-e10624-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0771/10905551/007d2a5a35e1/BTM2-9-e10624-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0771/10905551/ae59a73f7960/BTM2-9-e10624-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0771/10905551/5b9270fcb5d1/BTM2-9-e10624-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0771/10905551/4e32a57b50fa/BTM2-9-e10624-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0771/10905551/c7a63739544d/BTM2-9-e10624-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0771/10905551/a5ac7e02d8e3/BTM2-9-e10624-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0771/10905551/007d2a5a35e1/BTM2-9-e10624-g006.jpg

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本文引用的文献

1
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Adv Sci (Weinh). 2022 Feb;9(4):e2103999. doi: 10.1002/advs.202103999. Epub 2021 Dec 16.
2
Patient-derived organoids as a preclinical platform for precision medicine in colorectal cancer.患者来源的类器官作为结直肠癌精准医学的临床前平台。
Mol Oncol. 2022 Jun;16(12):2396-2412. doi: 10.1002/1878-0261.13144. Epub 2022 Jan 1.
3
Scientific Validation and Clinical Application of Lung Cancer Organoids.
微流控芯片在原发性泌尿系统癌症中的应用：最新进展与未来展望
Smart Med. 2025 May 19;4(2):e70010. doi: 10.1002/smmd.70010. eCollection 2025 Jun.
肺癌类器官的科学验证和临床应用。
Cells. 2021 Nov 4;10(11):3012. doi: 10.3390/cells10113012.
4
Organoid Sensitivity Correlates with Therapeutic Response in Patients with Pancreatic Cancer.类器官的敏感性与胰腺癌患者的治疗反应相关。
Clin Cancer Res. 2022 Feb 15;28(4):708-718. doi: 10.1158/1078-0432.CCR-20-4116.
5
The 2021 Updated European Association of Urology Guidelines on Metastatic Urothelial Carcinoma.2021 年更新版欧洲泌尿外科学会转移性尿路上皮癌临床实践指南。
Eur Urol. 2022 Jan;81(1):95-103. doi: 10.1016/j.eururo.2021.09.026. Epub 2021 Nov 3.
6
Presence of complete murine viral genome sequences in patient-derived xenografts.患者来源的异种移植中存在完整的鼠类病毒基因组序列。
Nat Commun. 2021 Apr 1;12(1):2031. doi: 10.1038/s41467-021-22200-5.
7
3D Collagen Vascular Tumor-on-a-Chip Mimetics for Dynamic Combinatorial Drug Screening.3D 胶原血管肿瘤芯片模拟物用于动态组合药物筛选。
Mol Cancer Ther. 2021 Jun;20(6):1210-1219. doi: 10.1158/1535-7163.MCT-20-0880. Epub 2021 Mar 30.
8
Organoid and Spheroid Tumor Models: Techniques and Applications.类器官和球体肿瘤模型：技术与应用
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9
Creation of bladder assembloids mimicking tissue regeneration and cancer.模拟组织再生和癌症的膀胱组装体的创建。
Nature. 2020 Dec;588(7839):664-669. doi: 10.1038/s41586-020-3034-x. Epub 2020 Dec 16.
10
Human organoids: model systems for human biology and medicine.人类类器官：人类生物学和医学的模型系统。
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