Kalita Bhaskar Jyoti, Sit Nandan
Department of Food Engineering and Technology, Tezpur University, Tezpur, Assam 784028 India.
Food Sci Biotechnol. 2023 Sep 4;33(5):1163-1175. doi: 10.1007/s10068-023-01422-x. eCollection 2024 Apr.
In the present study, cellulase has been immobilized by two different methods of entrapment viz. encapsulation in calcium alginate and matrix entrapment in agar. The calcium alginate encapsulated beads showed an immobilization efficiency of 92.11% and agar entrapped cubes showed an immobilization efficiency of 97.63%. The free cellulase was found to show optimum activity at 50 °C and pH 4, had a K of 39.29 mg/mL, V of 0.50 μmol/min. The calcium alginate encapsulated beads showed optimum activity at 50 °C, and pH 8, had a K of 72.28 mg/mL and V of 1.32 μmol/min. The agar entrapped cubes showed optimum activity at 60 °C, and pH 4, had a K of 13.08 mg/mL, and V of 0.38 μmol/min. The immobilized cellulases could be used for 5 cycles after which their activity deteriorated. The immobilized as well as the free enzyme were effective in increasing the yield of carrot juice.
在本研究中，纤维素酶已通过两种不同的包埋方法固定化，即包埋于海藻酸钙中以及包埋于琼脂的基质中。海藻酸钙包埋的珠子显示出92.11%的固定化效率，而琼脂包埋的立方体显示出97.63%的固定化效率。发现游离纤维素酶在50°C和pH 4时表现出最佳活性，K为39.29 mg/mL，V为0.50 μmol/min。海藻酸钙包埋的珠子在50°C和pH 8时表现出最佳活性，K为72.28 mg/mL，V为1.32 μmol/min。琼脂包埋的立方体在60°C和pH 4时表现出最佳活性，K为13.08 mg/mL，V为0.38 μmol/min。固定化纤维素酶可使用5个循环，之后其活性下降。固定化酶和游离酶均能有效提高胡萝卜汁的产量。
