i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, Porto, 4200-135, Portugal.
INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen, 208, Porto, 4200-135, Portugal.
Chem Soc Rev. 2024 Apr 22;53(8):3714-3773. doi: 10.1039/d3cs00921a.
Self-assembled monolayers (SAMs) represent highly ordered molecular materials with versatile biochemical features and multidisciplinary applications. Research on SAMs has made much progress since the early begginings of Au substrates and alkanethiols, and numerous examples of peptide-displaying SAMs can be found in the literature. Peptides, presenting increasing structural complexity, stimuli-responsiveness, and biological relevance, represent versatile functional components in SAMs-based platforms. This review examines the major findings and progress made on the use of peptide building blocks displayed as part of SAMs with specific functions, such as selective cell adhesion, migration and differentiation, biomolecular binding, advanced biosensing, molecular electronics, antimicrobial, osteointegrative and antifouling surfaces, among others. Peptide selection and design, functionalisation strategies, as well as structural and functional characteristics from selected examples are discussed. Additionally, advanced fabrication methods for dynamic peptide spatiotemporal presentation are presented, as well as a number of characterisation techniques. All together, these features and approaches enable the preparation and use of increasingly complex peptide-based SAMs to mimic and study biological processes, and provide convergent platforms for high throughput screening discovery and validation of promising therapeutics and technologies.
自组装单分子层（SAMs）是具有多种生化特性和多学科应用的高度有序分子材料。自 Au 基底和烷硫醇问世以来，SAMs 的研究取得了很大进展，文献中也有许多展示肽的 SAMs 的例子。肽具有越来越复杂的结构、刺激响应性和生物学相关性，是 SAMs 基平台中多功能的功能组件。本文综述了在使用作为 SAMs 一部分的肽构建块方面的主要发现和进展，这些肽具有特定的功能，如选择性细胞黏附、迁移和分化、生物分子结合、高级生物传感、分子电子学、抗菌、骨整合和抗污表面等。讨论了肽的选择和设计、功能化策略以及从选定示例中得到的结构和功能特征。此外，还介绍了用于动态肽时空呈现的先进制造方法以及一些特征分析技术。总之，这些特性和方法使得制备和使用越来越复杂的基于肽的 SAMs 成为可能，以模拟和研究生物过程，并为高通量筛选发现和验证有前途的治疗方法和技术提供了汇聚平台。
