São Carlos Institute of Physics, University of São Paulo, São Carlos, SP, Brazil.
Department of Animal Science, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, SP, Brazil.
Sci Rep. 2024 Mar 8;14(1):5715. doi: 10.1038/s41598-024-55574-9.
Kidney transplantation is a common yet highly demanding medical procedure worldwide, enhancing the quality of life for patients with chronic kidney disease. Despite its prevalence, the procedure faces a shortage of available organs, partly due to contamination by microorganisms, leading to significant organ disposal. This study proposes utilizing photonic techniques associated with organ support machines to prevent patient contamination during kidney transplantation. We implemented a decontamination system using ultraviolet-C (UV-C) irradiation on the preservation solution circulating through pigs' kidneys between harvest and implant. UV-C irradiation, alone or combined with ultrasound (US) and Ps80 detergent during ex-vivo swine organ perfusion in a Lifeport® Kidney Transporter machine, aimed to reduce microbiological load in both fluid and organ. Results show rapid fluid decontamination compared to microorganism release from the organ, with notable retention. By including Ps80 detergent at 0.5% during UV-C irradiation 3 log (CFU mL) of Staphylococcus aureus bacteria previously retained in the organ were successfully removed, indicating the technique's feasibility and effectiveness.
肾移植是全球范围内一种常见但要求极高的医疗程序，可提高慢性肾病患者的生活质量。尽管该程序很普遍，但由于微生物污染，可供使用的器官仍然短缺，这导致大量器官被丢弃。本研究提出利用与器官支持机器相关的光子技术来防止肾移植过程中患者受到污染。我们在猪肾从收获到植入过程中循环的保存液中实施了一种使用紫外线-C（UV-C）照射的去污系统。在 Lifeport® Kidney Transporter 机器中对离体猪器官进行灌注时，单独使用 UV-C 照射或与超声（US）和 Ps80 清洁剂联合使用，旨在减少液体和器官中的微生物负荷。结果表明，与从器官中释放的微生物相比，液体的去污速度更快，且保留效果显著。通过在 UV-C 照射时加入 0.5%的 Ps80 清洁剂，成功去除了先前保留在器官中的 3 对数（CFU mL）金黄色葡萄球菌细菌，表明该技术的可行性和有效性。
