Enzyme and Microbial Technology Research Centre, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Department of Biochemistry, FacultyofBiotechnologyand BiomolecularSciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Arch Microbiol. 2024 Mar 12;206(4):152. doi: 10.1007/s00203-024-03871-2.
Producing recombinant proteins is a major accomplishment of biotechnology in the past century. Heterologous hosts, either eukaryotic or prokaryotic, are used for the production of these proteins. The utilization of microbial host systems continues to dominate as the most efficient and affordable method for biotherapeutics and food industry productions. Hence, it is crucial to analyze the limitations and advantages of microbial hosts to enhance the efficient production of recombinant proteins on a large scale. E. coli is widely used as a host for the production of recombinant proteins. Researchers have identified certain obstacles with this host, and given the growing demand for recombinant protein production, there is an immediate requirement to enhance this host. The following review discusses the elements contributing to the manifestation of recombinant protein. Subsequently, it sheds light on innovative approaches aimed at improving the expression of recombinant protein. Lastly, it delves into the obstacles and optimization methods associated with translation, mentioning both cis-optimization and trans-optimization, producing soluble recombinant protein, and engineering the metal ion transportation. In this context, a comprehensive description of the distinct features will be provided, and this knowledge could potentially enhance the expression of recombinant proteins in E. coli.
生产重组蛋白是过去一个世纪生物技术的主要成就。无论是真核生物还是原核生物，异源宿主都被用于这些蛋白的生产。由于微生物宿主系统是生物制药和食品工业生产最有效和最经济的方法，因此它仍然占据主导地位。因此，分析微生物宿主的局限性和优势对于大规模高效生产重组蛋白至关重要。大肠杆菌被广泛用作生产重组蛋白的宿主。研究人员已经确定了这个宿主存在的某些障碍，并且鉴于对重组蛋白生产的需求不断增长，立即需要增强这个宿主。以下综述讨论了影响重组蛋白表达的因素。随后，它介绍了旨在提高重组蛋白表达的创新方法。最后，它探讨了与翻译相关的障碍和优化方法，提到了顺式优化和反式优化、生产可溶性重组蛋白以及工程金属离子运输。在这种情况下，将提供对不同特征的全面描述，这些知识可能会增强大肠杆菌中重组蛋白的表达。
