Jaiswal Namrata, Chanda Bidisha, Gilliard Andrea, Shi Ainong, Ling Kai-Shu
United States Department of Agriculture, Agricultural Research Service, U.S. Vegetable Laboratory, Charleston, SC 29414, USA.
Department of Horticulture, University of Arkansas, Fayetteville, AR 72701, USA.
Plants (Basel). 2024 Feb 21;13(5):581. doi: 10.3390/plants13050581.
The tomato is one of the most important vegetable crops grown worldwide. Tomato brown rugose fruit virus (ToBRFV), a seed-borne tobamovirus, poses a serious threat to tomato production due to its ability to break the resistant genes (, , ) in tomatoes. The objective of this work was to identify new resistant source(s) of tomato germplasm against ToBRFV. To achieve this aim, a total of 476 accessions from 12 species were tested with the ToBRFV US isolate for their resistance and susceptibility. As a result, a total of 44 asymptomatic accessions were identified as resistant/tolerant, including thirty-one accessions of , one accession of , four accessions of , three accessions of and five accessions of subsection hybrid. Further analyses using serological tests identified four highly resistant lines, PI 390713, PI 390714, PI 390716, and PI 390717. The inheritance of resistance in the selected lines was verified in the next generation and confirmed using RT-qPCR. To our knowledge, this is a first report of high resistance to ToBRFV in . These new genetic resources will expand the genetic pool available for breeders to develop new resistant cultivars of tomato against ToBRFV.
番茄是全球种植的最重要蔬菜作物之一。番茄褐色皱纹果病毒（ToBRFV）是一种种传烟草花叶病毒，因其能够突破番茄中的抗性基因（……），对番茄生产构成严重威胁。这项工作的目的是鉴定番茄种质对ToBRFV的新抗性来源。为实现这一目标，用ToBRFV美国分离株对来自12个物种的总共476份种质进行了抗性和敏感性测试。结果，共鉴定出44份无症状种质为抗性/耐受性种质，包括31份……种质、1份……种质、4份……种质、3份……种质和5份……杂交亚组种质。进一步使用血清学测试分析鉴定出4个高抗……品系，即PI 390713、PI 390714、PI 390716和PI 390717。所选品系的抗性遗传在下一代中得到验证，并通过RT-qPCR得到确认。据我们所知，这是……对ToBRFV具有高抗性的首次报道。这些新的遗传资源将扩大可供育种者利用的基因库，以培育抗ToBRFV的番茄新品种。
