School of Marine Science, Ningbo University, Ningbo, Zhejiang 315000, China.
Key Laboratory of Aquacultral Biotechnology Ministry of Education, Ningbo University, Ningbo, Zhejiang 315000, China.
J Agric Food Chem. 2024 Mar 27;72(12):6585-6592. doi: 10.1021/acs.jafc.3c08435. Epub 2024 Mar 17.
The advantages of farming in saline-alkali water have gradually attracted attention, but few studies have focused on its flavor. In this study, cultured in saline-alkali water (SS) and ordinary seawater (CS) (both have a breeding time of 120 days) were selected for analysis ( = 5). High-performance liquid chromatography (HPLC) was used to measure free amino acids and flavoring nucleotides in the muscles of , while the taste activity value (TAV) and equivalent umami concentration (EUC) were used to analyze the degree of umami. The total essential amino acids (TEAA) in the SS group were 238.41 ± 46.24 mg/mL, significantly higher than that in the CS group (107.06 ± 15.65 mg/mL). The total amount of flavor nucleotides in the SS group was 2948.51 ± 233.66 μg/mL, significantly higher than those in the CS group (2530.37 ± 114.67 μg/mL). The content and TAV of some free amino acids (Glu, Cys-s) in the SS group were significantly higher. Meanwhile, due to the significant increase in IMP, the synergistic effect of free amino acids and flavored nucleotides leads to higher EUC. The significant separation of SS and CS samples in principal component analysis (PCA) indicates a significant difference between the two groups. Our results indicate that shrimp cultured in saline-alkali water has a stronger umami. This study enriches the basic theories related to the flavor of salt-alkali water crustaceans.
养殖在盐碱水中的优势逐渐受到关注,但很少有研究关注其风味。本研究选择在盐碱水(SS)和普通海水(CS)中培养 120 天的 (n = 5)进行分析。采用高效液相色谱法(HPLC)测定肌肉中的游离氨基酸和呈味核苷酸,用味觉活性值(TAV)和等效鲜味浓度(EUC)分析鲜味程度。SS 组的总必需氨基酸(TEAA)为 238.41 ± 46.24 mg/mL,明显高于 CS 组(107.06 ± 15.65 mg/mL)。SS 组的总呈味核苷酸含量为 2948.51 ± 233.66 μg/mL,明显高于 CS 组(2530.37 ± 114.67 μg/mL)。SS 组部分游离氨基酸(Glu、Cys-s)的含量和 TAV 明显较高。同时,由于 IMP 的显著增加,游离氨基酸和呈味核苷酸的协同作用导致更高的 EUC。主成分分析(PCA)中 SS 和 CS 样品的显著分离表明两组间存在显著差异。研究结果表明,在盐碱水中养殖的虾具有更强的鲜味。本研究丰富了盐碱水甲壳类动物风味的基础理论。