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综合分析荞麦(苦荞麦)的胚胎学。

Comprehensive Embryological Analyses of Common Buckwheat (Fagopyrym esculentum Moench).

机构信息

Department of Plant Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian University in Kraków, Cracow, Poland.

Department of Physiology, Breeding of Plant and Seed Science, University of Agriculture, Cracow, Poland.

出版信息

Methods Mol Biol. 2024;2791:97-105. doi: 10.1007/978-1-0716-3794-4_10.

Abstract

Knowledge of detailed reproductive biology of cultivated species is important as requirements for fruit and seed production allow the development of effective management strategies and a sustainable use. Embryological processes of common buckwheat (Fagopyrum esculentum Moench) are difficult to interpret due to the influence of genetic determinants, i.e., dimorphic heterostyly resulting in the production of long- and short-styled flowers, and environmental predisposition, i.e., sensitivity of ovules to thermal stress. Furthermore, the situation is complicated by overproduction of flowers and depletion of resources as the plant ages. Herein we provide protocols that allow to visualize both basic and more specific embryological features and also disturbances in sexual reproduction of common buckwheat resulting from external and internal factors. All stages of plant material fixation, preparation, staining, and observation are described and explained in detail. Technical tips and pictures of properly prepared microscopic sections are also provided.

摘要

了解栽培物种的详细生殖生物学很重要,因为果实和种子生产的要求可以制定出有效的管理策略和可持续的利用方案。普通荞麦(Fagopyrum esculentum Moench)的胚胎发育过程由于遗传决定因素(即产生长花柱和短花柱花的二形异型雄雌同花)和环境倾向(即胚珠对热胁迫的敏感性)的影响而难以解释。此外,随着植物年龄的增长,花朵过度产生和资源消耗的情况会使情况变得更加复杂。本文提供了可以可视化普通荞麦基本和更具体的胚胎发育特征以及由内外因素引起的有性生殖障碍的方案。植物材料固定、准备、染色和观察的所有阶段都进行了详细描述和解释。还提供了正确准备的显微镜切片的技术提示和图片。

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