School of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, Guangzhou, 510650, China.
World J Microbiol Biotechnol. 2024 Mar 27;40(5):144. doi: 10.1007/s11274-024-03957-5.
Proteases, enzymes that catalyze the hydrolysis of peptide bonds in proteins, are important in the food industry, biotechnology, and medical fields. With increasing demand for proteases, there is a growing emphasis on enhancing their expression and production through microbial systems. However, proteases' native hosts often fall short in high-level expression and compatibility with downstream applications. As a result, the recombinant production of proteases has become a significant focus, offering a solution to these challenges. This review presents an overview of the current state of protease production in prokaryotic and eukaryotic expression systems, highlighting key findings and trends. In prokaryotic systems, the Bacillus spp. is the predominant host for proteinase expression. Yeasts are commonly used in eukaryotic systems. Recent advancements in protease engineering over the past five years, including rational design and directed evolution, are also highlighted. By exploring the progress in both expression systems and engineering techniques, this review provides a detailed understanding of the current landscape of recombinant protease research and its prospects for future advancements.
蛋白酶是一种能够催化蛋白质中肽键水解的酶，在食品工业、生物技术和医学领域都有重要作用。随着对蛋白酶的需求不断增加，通过微生物系统来提高其表达和生产水平的重要性也日益凸显。然而，蛋白酶的天然宿主在高水平表达和与下游应用的兼容性方面往往存在不足。因此，重组蛋白酶的生产已经成为一个重要的关注点，为解决这些挑战提供了一种方案。
本文综述了原核和真核表达系统中蛋白酶生产的现状，重点介绍了关键发现和趋势。在原核系统中，芽孢杆菌属是蛋白水解酶表达的主要宿主。在真核系统中，酵母通常被使用。本文还强调了过去五年中蛋白酶工程方面的最新进展，包括理性设计和定向进化。
通过探索表达系统和工程技术方面的进展，本文详细了解了重组蛋白酶研究的现状及其未来发展的前景。
