黄芪总苷通过调节免疫微环境对糖尿病难愈创面的影响。
Effect of total astragalosides on diabetic non-healing wound by regulating immune microenvironment.
作者信息
Fan Weijing, Lu Huimin, Yuan Xin, Zhao Kun, Liu Guobin
机构信息
Vascular Surgery Department, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
College of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, China.
出版信息
Postepy Dermatol Alergol. 2024 Feb;41(1):100-105. doi: 10.5114/ada.2024.135989. Epub 2024 Feb 28.
AIM
The aim of the research was to analyse the regulatory effect of astragaloside (AST) on the immune microenvironment of diabetic non-healing wound (DNHW), and to analyse the clinical efficacy and mechanism of wound repair in multiple layers.
MATERIAL AND METHODS
Ninety adult male Wistar rats, which were kept healthy (SPF) under natural infection, were randomly divided into three groups, namely, blank, control and observation groups, with 30 rats in each group. After adaptive feeding for 7 days, the diabetes model was established. After the model was formed, the wounds were uniformly prepared, and then the blank group only was shaved. Both the control group and the observation group were treated with moist exposure therapy. The control group was covered with physiological saline gauze, while the observation group was covered with AST gauze. The healing status of the wounds in both groups was observed and recorded on the 1, 7, and 14 days after formation. And the levels of α-smooth muscle actin (α-SMA) and collagen I (COL-1) in the wound tissue were measured.
RESULTS
On the 1st day after wound formation, the wound healing area, α-SMA, and COL-1 levels in the three groups were consistent ( > 0.05). On the 7 and 14 days after wound formation, the wound healing area in the three groups increased compared within the group, but only the control and observation groups had significantly higher wound healing area than on the 1st day after wound formation ( < 0.05). In addition, the blank group had lower levels of α-SMA and COL-1, while the control and observation groups had higher levels of α-SMA and COL-1 ( < 0.05). In the comparison between groups, the wound healing area, α-SMA, and COL-1 levels in the control and observation groups were higher than those in the blank group, while the wound healing area, α-SMA, and COL-1 levels in the observation group were higher than those in the control group ( < 0.05).
CONCLUSIONS
AST can regulate the immune microenvironment of DNHW, improve α-SMA and COL-1, and accelerate the wound healing of DNHW.
目的
本研究旨在分析黄芪甲苷（AST）对糖尿病难愈性伤口（DNHW）免疫微环境的调节作用，并从多个层面分析其促进伤口修复的临床疗效及机制。
材料与方法
选取90只成年雄性Wistar大鼠，在自然感染条件下保持健康（SPF级），随机分为空白组、对照组和观察组，每组30只。适应性喂养7天后，建立糖尿病模型。模型建立后，统一制备伤口，然后仅对空白组进行剃毛处理。对照组和观察组均采用湿润暴露疗法治疗。对照组覆盖生理盐水纱布，观察组覆盖AST纱布。在伤口形成后的第1、7和14天观察并记录两组伤口的愈合情况。同时检测伤口组织中α平滑肌肌动蛋白（α-SMA）和Ⅰ型胶原蛋白（COL-1）的水平。
结果
伤口形成后第1天，三组的伤口愈合面积、α-SMA和COL-1水平一致（P>0.05）。伤口形成后第7天和第14天，三组内伤口愈合面积均较第1天增加，但仅对照组和观察组伤口愈合面积显著高于伤口形成后第1天（P<0.05）。此外，空白组α-SMA和COL-1水平较低，而对照组和观察组α-SMA和COL-1水平较高（P<0.05）。组间比较，对照组和观察组的伤口愈合面积、α-SMA和COL-1水平均高于空白组，而观察组的伤口愈合面积、α-SMA和COL-1水平高于对照组（P<0.05）。
结论
AST可调节DNHW的免疫微环境，提高α-SMA和COL-1水平，加速DNHW伤口愈合。
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