Department of Nervous and Muscular Physiology, Bogomoletz Institute of Physiology, Kiyv, Ukraine.
Physiol Rep. 2024 Mar;12(6):e15992. doi: 10.14814/phy2.15992.
Aorta, the largest vessel in the body, is generally considered anatomically homogeneous, yet spatial functional differences exist. In our study, we conducted a comprehensive analysis by reexamining public RNA-SEQ data, comparing expression patterns between thoracic and abdominal aorta. Additionally, we measured acetylcholine-induced relaxations of the different regions of thoracic aorta in Wistar Rats. Our results revealed a distinct percentage difference in acetylcholine-induced relaxation in the proximal and distal segments of the thoracic aorta (p = 1.14e-4). To explain this variation, we performed differential expression analysis of previously published RNA-sequencing data between thoracic and abdominal aorta, which showed 497 differentially expressed genes between these locations. From results of RNA-Seq analysis, we draw a hypothesis that differential expressions of the potassium inward rectifying channels (K) and voltage gated calcium channels (VGCC) presumably located on SMC, with higher expression in the distal thoracic segments in comparison with the proximal thoracic segments of aorta, can explain differences in acetylcholine-induced relaxation. Notably, specific blockade of K eliminated differences between the proximal and distal regions of thoracic aorta, underscoring their significance in understanding the spatial nuances in aortic behavior, also blockade of VGCC, shows a higher effect on basal tone, in distal region of thoracic aorta in comparison with proximal.
主动脉是人体中最大的血管，通常被认为在解剖学上是均匀的，但存在空间功能差异。在我们的研究中，我们通过重新检查公共 RNA-SEQ 数据，比较胸主动脉和腹主动脉之间的表达模式，进行了全面的分析。此外，我们还测量了 Wistar 大鼠胸主动脉不同区域对乙酰胆碱诱导的舒张反应。我们的结果显示，胸主动脉近端和远端段对乙酰胆碱诱导的舒张反应存在明显的百分比差异（p=1.14e-4）。为了解释这种变异，我们对胸主动脉和腹主动脉之间先前发表的 RNA 测序数据进行了差异表达分析，结果显示这两个部位有 497 个差异表达基因。从 RNA-Seq 分析的结果中，我们提出了一个假设，即钾内向整流通道（K）和电压门控钙通道（VGCC）的差异表达可能位于 SMC 上，与胸主动脉的近端相比，远端胸段的表达更高，这可以解释乙酰胆碱诱导的舒张反应的差异。值得注意的是，K 的特异性阻断消除了胸主动脉近端和远端之间的差异，突显了它们在理解主动脉行为的空间细微差别方面的重要性，VGCC 的阻断也显示出在胸主动脉远端对基础张力的影响更高，与近端相比。
