文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

调制式电温热疗增强载多柔比星的溶酶体热敏脂质体在 4T1 荷瘤小鼠中的肿瘤递送并提高抗癌活性。

Modulated Electro-Hyperthermia Accelerates Tumor Delivery and Improves Anticancer Activity of Doxorubicin Encapsulated in Lyso-Thermosensitive Liposomes in 4T1-Tumor-Bearing Mice.

机构信息

Institute of Translational Medicine, Semmelweis University, 1094 Budapest, Hungary.

HUN-REN-SU Cerebrovascular and Neurocognitive Diseases Research Group, 1094 Budapest, Hungary.

出版信息

Int J Mol Sci. 2024 Mar 7;25(6):3101. doi: 10.3390/ijms25063101.

DOI:10.3390/ijms25063101
PMID:38542073
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10970314/
Abstract

Modulated electro-hyperthermia (mEHT) is an adjuvant cancer therapy that enables tumor-selective heating (+2.5 °C). In this study, we investigated whether mEHT accelerates the tumor-specific delivery of doxorubicin (DOX) from lyso-thermosensitive liposomal doxorubicin (LTLD) and improves its anticancer efficacy in mice bearing a triple-negative breast cancer cell line (4T1). The 4T1 cells were orthotopically injected into Balb/C mice, and mEHT was performed on days 9, 12, and 15 after the implantation. DOX, LTLD, or PEGylated liposomal DOX (PLD) were administered for comparison. The tumor size and DOX accumulation in the tumor were measured. The cleaved caspase-3 (cC3) and cell proliferation were evaluated by cC3 or Ki67 immunohistochemistry and Western blot. The LTLD+mEHT combination was more effective at inhibiting tumor growth than the free DOX and PLD, demonstrated by reductions in both the tumor volume and tumor weight. LTLD+mEHT resulted in the highest DOX accumulation in the tumor one hour after treatment. Tumor cell damage was associated with cC3 in the damaged area, and with a reduction in Ki67 in the living area. These changes were significantly the strongest in the LTLD+mEHT-treated tumors. The body weight loss was similar in all mice treated with any DOX formulation, suggesting no difference in toxicity. In conclusion, LTLD combined with mEHT represents a novel approach for DOX delivery into cancer tissue.

摘要

调制的电加热疗法（mEHT）是一种辅助癌症治疗方法，可实现肿瘤选择性加热（+2.5°C）。在这项研究中，我们研究了 mEHT 是否能加速溶酶体热敏脂质体阿霉素（LTLD）中阿霉素（DOX）的肿瘤特异性释放，并提高携带三阴性乳腺癌细胞系（4T1）的小鼠的抗癌疗效。将 4T1 细胞原位注射到 Balb/C 小鼠中，并在植入后第 9、12 和 15 天进行 mEHT。进行 DOX、LTLD 或聚乙二醇化脂质体 DOX（PLD）给药以进行比较。测量肿瘤大小和肿瘤内 DOX 积累。通过 cC3 或 Ki67 免疫组化和 Western blot 评估裂解的半胱天冬酶-3（cC3）和细胞增殖。LTLD+mEHT 组合比游离 DOX 和 PLD 更能有效抑制肿瘤生长，表现为肿瘤体积和肿瘤重量均降低。LTLD+mEHT 在治疗后一小时导致肿瘤内 DOX 积累最高。肿瘤细胞损伤与受损区域中的 cC3 相关，与活区中的 Ki67 减少相关。这些变化在 LTLD+mEHT 治疗的肿瘤中最强。所有接受任何 DOX 制剂治疗的小鼠的体重减轻相似，表明毒性无差异。总之，LTLD 与 mEHT 联合代表了一种将 DOX 递送到癌症组织的新方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/0b8b0688cc47/ijms-25-03101-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/906c23842da2/ijms-25-03101-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/64bd2c06d2fe/ijms-25-03101-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/70e0f0e734ff/ijms-25-03101-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/9a3d453acbac/ijms-25-03101-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/b8493296eab7/ijms-25-03101-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/9149ec858c34/ijms-25-03101-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/cdd8bfce2753/ijms-25-03101-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/aecfce07108e/ijms-25-03101-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/0b8b0688cc47/ijms-25-03101-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/906c23842da2/ijms-25-03101-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/64bd2c06d2fe/ijms-25-03101-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/70e0f0e734ff/ijms-25-03101-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/9a3d453acbac/ijms-25-03101-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/b8493296eab7/ijms-25-03101-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/9149ec858c34/ijms-25-03101-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/cdd8bfce2753/ijms-25-03101-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/aecfce07108e/ijms-25-03101-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfb4/10970314/0b8b0688cc47/ijms-25-03101-g009.jpg

相似文献

[1]
Modulated Electro-Hyperthermia Accelerates Tumor Delivery and Improves Anticancer Activity of Doxorubicin Encapsulated in Lyso-Thermosensitive Liposomes in 4T1-Tumor-Bearing Mice.

Int J Mol Sci. 2024-3-7

[2]
Lyso-thermosensitive liposomal doxorubicin for treatment of bladder cancer.

Int J Hyperthermia. 2017-5-10

[3]
The effect of injected dose on localized tumor accumulation and cardiac uptake of doxorubicin in a Vx2 rabbit tumor model using MR-HIFU mild hyperthermia and thermosensitive liposomes.

Int J Hyperthermia. 2020

[4]
Modulated electro-hyperthermia induced p53 driven apoptosis and cell cycle arrest additively support doxorubicin chemotherapy of colorectal cancer in vitro.

Cancer Med. 2019-6-10

[5]
Modulated electro-hyperthermia-enhanced liposomal drug uptake by cancer cells.

Int J Nanomedicine. 2019-2-18

[6]
Short-time focused ultrasound hyperthermia enhances liposomal doxorubicin delivery and antitumor efficacy for brain metastasis of breast cancer.

Int J Nanomedicine. 2014-9-19

[7]
Focused Ultrasound Hyperthermia Mediated Drug Delivery Using Thermosensitive Liposomes and Visualized With Two-Photon Microscopy.

Theranostics. 2017-7-7

[8]
Ivermectin Synergizes with Modulated Electro-hyperthermia and Improves Its Anticancer Effects in a Triple-Negative Breast Cancer Mouse Model.

ACS Pharmacol Transl Sci. 2024-7-17

[9]
Pharmacokinetics, Tissue Distribution and Therapeutic Effect of Cationic Thermosensitive Liposomal Doxorubicin Upon Mild Hyperthermia.

Pharm Res. 2016-3

[10]
Drug development of lyso-thermosensitive liposomal doxorubicin: Combining hyperthermia and thermosensitive drug delivery.

Adv Drug Deliv Rev. 2021-11

引用本文的文献

[1]
A Comprehensive Review of Smart Thermosensitive Nanocarriers for Precision Cancer Therapy.

Int J Mol Sci. 2025-7-29

[2]
Ivermectin Synergizes with Modulated Electro-hyperthermia and Improves Its Anticancer Effects in a Triple-Negative Breast Cancer Mouse Model.

ACS Pharmacol Transl Sci. 2024-7-17

本文引用的文献

[1]
The Clinical Validation of Modulated Electro-Hyperthermia (mEHT).

Cancers (Basel). 2023-9-15

[2]
Hyperthermia combined with chemotherapy chemotherapy in patients with advanced pancreatic cancer: A multicenter retrospective observational comparative study.

World J Clin Oncol. 2023-6-24

[3]
Recent Preclinical and Clinical Progress in Liposomal Doxorubicin.

Pharmaceutics. 2023-3-9

[4]
Concomitant delivery of doxorubicin and cisplatin through liposome-based thermosensitive nanoparticles: perspective in the treatment of cancer in animal models.

Am J Cancer Res. 2023-2-15

[5]
Doxorubicin-An Agent with Multiple Mechanisms of Anticancer Activity.

Cells. 2023-2-19

[6]
Re-purposing the pro-senescence properties of doxorubicin to introduce immunotherapy in breast cancer brain metastasis.

Cell Rep Med. 2022-11-15

[7]
Effects of Modulated Electro-Hyperthermia (mEHT) on Two and Three Year Survival of Locally Advanced Cervical Cancer Patients.

Cancers (Basel). 2022-1-27

[8]
Mild hyperthermia accelerates doxorubicin clearance from tumour-extravasated temperature-sensitive liposomes.

Nanotheranostics. 2022

[9]
Turning down the heat: The case for mild hyperthermia and thermosensitive liposomes.

Nanomedicine. 2022-2

[10]
Drug development of lyso-thermosensitive liposomal doxorubicin: Combining hyperthermia and thermosensitive drug delivery.

Adv Drug Deliv Rev. 2021-11

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索