Department of Chemistry and Biology, Graduate School of Science and Engineering, Ehime University, 2-5 Bunkyo, Matsuyama 790-8577, Ehime, Japan.
Department of Chemistry, Faculty of Science, Ehime University, 2-5 Bunkyo, Matsuyama 790-8577, Ehime, Japan.
Int J Mol Sci. 2024 Mar 7;25(6):3112. doi: 10.3390/ijms25063112.
Protein amyloids have attracted attention for their application as functional amyloid materials because of their strong properties, such as high resistance to chemical or biological degradation, despite their medical issues. Amyloids can be used for various applications by modifying the amyloid surface with functional materials, such as proteins and polymers. In this study, we investigated the effect of polyallylamine (PAA), a functional cationic polymer as a candidate for amyloid modification, on the amyloids formed from amyloid β (Aβ) peptide. It was demonstrated for the first time that PAA can bind to Aβ amyloids through fluorescence observations and the quenched emission from the tyrosine at site 10 near the fibrillogenic core. These results suggest that PAA could be used to develop new functional amyloids. However, notably, coating Aβ amyloid with PAA could affect conventional amyloid detection assays such as thioflavin T assay and detection using antibodies. Thus, our results also indicate that consideration would be necessary for the analysis of functional amyloids coated with various polymers.
蛋白质淀粉样纤维因其高强度特性而备受关注，可作为功能性淀粉样物质应用，尽管存在一些医学问题。通过在淀粉样表面修饰功能性材料（如蛋白质和聚合物），可以将淀粉样纤维用于各种应用。在这项研究中，我们研究了聚烯丙胺（PAA）作为淀粉样纤维修饰候选物对淀粉样β（Aβ）肽形成的淀粉样纤维的影响。首次证明 PAA 可以通过荧光观察和纤维原核心附近 10 位酪氨酸的荧光猝灭来结合 Aβ 淀粉样纤维。这些结果表明，PAA 可用于开发新的功能性淀粉样纤维。然而，值得注意的是，用 PAA 包裹 Aβ 淀粉样纤维可能会影响传统的淀粉样检测分析，如硫黄素 T 检测和使用抗体的检测。因此，我们的结果还表明，对于用各种聚合物包裹的功能性淀粉样纤维的分析，需要考虑到这一点。
Zotero 插件
