液相色谱-串联质谱法分析印度尼西亚食品中的羧甲基赖氨酸。

Liquid Chromatography with Tandem Mass Spectrometry Analysis of Carboxymethyl Lysine in Indonesian Foods.

机构信息

Department of Nutrition, Faculty of Medicine, Universitas Trisakti, Jakarta 11440, Indonesia.

Nutrition Study Center, Faculty of Medicine, Universitas Trisakti, Jakarta 11440, Indonesia.

出版信息

Molecules. 2024 Mar 15;29(6):1304. doi: 10.3390/molecules29061304.

Abstract

There is little data on directly measured carboxymethyl lysine (CML) content in Indonesian foods. This study aimed to generate a database of CML values in foods commonly consumed in West Java and West Sumatra. The results were to be used to update our previous estimated CML values. CML values in food samples were measured using high-pressure liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). Food protein content was analyzed by Kjeldahl's method or inferred from the nutrition facts' label. A total of 210 food samples were examined, with the food groups of meat and poultry (1.06 mg CML/100 g edible food), and starchy foods (0.21 mg/100 g edible food) having the highest and lowest mean CML levels, respectively. We found that the foods with the top three highest CML content were fried starch dough (), fried fish crackers, and chicken The mean of the estimated values (0.80 mg CML/100 g edible food) was higher than the directly measured values (0.66 mg CML/100 g edible food), [ < 0.035]. Conclusion: This database provides information on CML values in Indonesian foods, and can be further used to make a guide policy for the selection of foods to reduce non-communicable diseases. Further measurements are needed on Indonesian dishes to complete the database.

摘要

印度尼西亚食品中羧甲基赖氨酸(CML)含量的直接测量数据很少。本研究旨在生成西爪哇省和西苏门答腊省常见食品中 CML 值的数据库。研究结果将用于更新我们之前的 CML 估计值。使用高效液相色谱-串联质谱法(HPLC-MS/MS)测量食品样品中的 CML 值。采用凯氏定氮法或从营养成分标签中推断出食品蛋白质含量。共检测了 210 种食品样品,其中肉类和家禽(1.06mg CML/100g 可食用食品)和淀粉类食品(0.21mg/100g 可食用食品)的 CML 水平最高和最低。我们发现 CML 含量最高的三种食物是炸淀粉面团()、炸鱼饼干和鸡肉。估计值的平均值(0.80mg CML/100g 可食用食品)高于直接测量值(0.66mg CML/100g 可食用食品),[<0.035]。结论:该数据库提供了印度尼西亚食品中 CML 值的信息,并可进一步用于制定减少非传染性疾病的食物选择指南。需要对印度尼西亚菜肴进行进一步测量以完成数据库。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d260/10975859/b7c51a2eab7e/molecules-29-01304-g001.jpg

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