中文译文：捷克葡萄藤中科病毒的多重感染。

Multiple Infections with Viruses of the Family in Czech Grapevines.

机构信息

Ecology, Diagnostics and Genetic Resources of Agriculturally Important Viruses, Fungi and Phytoplasmas, Crop Research Institute, Drnovská 507, 161 06 Prague, Czech Republic.

Department of Plant Protection, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague, Czech Republic.

出版信息

Viruses. 2024 Feb 23;16(3):343. doi: 10.3390/v16030343.

DOI:10.3390/v16030343

PMID:38543709

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10975331/

Abstract

This study focused on the viruses of the family that infect grapevines in the Czech Republic. Complete sequences of GFkV (grapevine fleck virus) and GRGV (grapevine red globe virus) from the genus and GRVFV (grapevine rupestris vein feathering virus) and GSyV-1 (grapevine Syrah virus 1) from the genus were obtained using high-throughput sequencing of small RNAs and total RNAs. Mixed infections with these viruses were observed, as well as several variants of these viruses in the same plant. Phylogenetic analysis showed the position of the newly obtained virus isolates within the family. Recombinant analysis provided evidence of single and multiple intraspecific recombinations in GRGV, GSyV-1, and GRVFV. Additionally, GAMaV, a grapevine virus from the genus , was reported for the first time in the Czech Republic.

摘要

本研究关注的是在捷克共和国感染葡萄树的病毒家族。使用高通量测序小 RNA 和总 RNA 的方法，获得了病毒属的 GFkV（葡萄斑驳病毒）和 GRGV（葡萄红地球病毒）以及病毒属的 GRVFV（葡萄卷叶病毒）和 GSyV-1（葡萄 Syrah 病毒 1）的完整序列。观察到这些病毒的混合感染，以及同一植物中这些病毒的多个变体。系统发育分析显示新获得的病毒分离株在病毒科内的位置。重组分析提供了 GRGV、GSyV-1 和 GRVFV 中单个和多个种内重组的证据。此外，还首次在捷克共和国报道了病毒属的 GAMaV。