Zang Yu, Li Zhenyang, Lu Yuxin, Hang Jiawei, Yuan Wei, Sun Jie
College of Biological Engineering, Zhejiang University of Technology, Huzhou 313000, Zhejiang, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Mar 25;40(3):847-857. doi: 10.13345/j.cjb.230348.
Bisabolene is a compound commonly found in essential oils of various plants. It has a broad application in sectors such as chemical, pharmaceutical, and health-care products. This study focuses on modifying the glycerol metabolism pathway to obtain a high bisabolene-producing strain of . To achieve this, the glycerol transporter gene from and the glycerol dehydrogenase gene from were overexpressed in engineered yeast YS036, which was equipped with a promoters-enhanced mevalonic acid pathway. Additionally, the glucose-inhibiting transcription factor was knocked out to reduce glucose inhibition. The results showed that the promoter transcription levels of the recombinant yeast strains increased, and the co-utilization of sucrose and glycerol was further improved in -knockout strain. Moreover, the maximum yield of bisabolene in shaking flask fermentation increased to 866.7 mg/L, an 82.2% increase compared to that of the original strain. By modifying the metabolic pathway of carbon sources, the yield of bisabolene was considerably improved. This study offers an effective strategy for enhancing the yield of terpene compounds in engineered yeast.
没药烯是一种常见于各种植物精油中的化合物。它在化工、制药和保健品等领域有着广泛应用。本研究聚焦于改造甘油代谢途径以获得高产没药烯的菌株。为此，来自[具体物种1]的甘油转运蛋白基因和来自[具体物种2]的甘油脱氢酶基因在配备了增强型甲羟戊酸途径启动子的工程酵母YS036中过表达。此外，敲除了葡萄糖抑制转录因子以减少葡萄糖抑制作用。结果表明，重组酵母菌株的[具体启动子名称]启动子转录水平提高，在敲除[具体转录因子名称]的菌株中蔗糖和甘油的共利用进一步改善。而且，摇瓶发酵中没药烯的最大产量提高到866.7毫克/升，与原始菌株相比增加了82.2%。通过改造碳源代谢途径，没药烯的产量得到显著提高。本研究为提高工程酵母中萜类化合物产量提供了一种有效策略。
