[Enhancing the glycerol utilization of engineered yeast increases its bisabolene production].

Bisabolene is a compound commonly found in essential oils of various plants. It has a broad application in sectors such as chemical, pharmaceutical, and health-care products. This study focuses on modifying the glycerol metabolism pathway to obtain a high bisabolene-producing strain of . To achieve this, the glycerol transporter gene from and the glycerol dehydrogenase gene from were overexpressed in engineered yeast YS036, which was equipped with a promoters-enhanced mevalonic acid pathway. Additionally, the glucose-inhibiting transcription factor was knocked out to reduce glucose inhibition. The results showed that the promoter transcription levels of the recombinant yeast strains increased, and the co-utilization of sucrose and glycerol was further improved in -knockout strain. Moreover, the maximum yield of bisabolene in shaking flask fermentation increased to 866.7 mg/L, an 82.2% increase compared to that of the original strain. By modifying the metabolic pathway of carbon sources, the yield of bisabolene was considerably improved. This study offers an effective strategy for enhancing the yield of terpene compounds in engineered yeast.