生成类似于 8-16 细胞期胚胎细胞的小鼠桑椹胚样细胞的方案。

Protocol for generating mouse morula-like cells resembling 8- to 16-cell stage embryo cells.

机构信息

Guangzhou Medical University, Panyu District, Guangzhou 511495, Guangdong Province, China; Guangzhou National Laboratory, Guangzhou International Bio Island, Guangzhou 510005, Guangdong Province, China.

出版信息

STAR Protoc. 2024 Jun 21;5(2):102934. doi: 10.1016/j.xpro.2024.102934. Epub 2024 Mar 29.

DOI:10.1016/j.xpro.2024.102934

PMID:38555588

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10998243/

Abstract

Generating cell types with properties of embryo cells with full developmental potential is of great biological importance. Here, we present a protocol for generating mouse morula-like cells (MLCs) resembling 8- to 16-cell stage embryo cells. We describe steps for induction, via increasing Stat3 activation, and the isolation of MLCs. We then detail procedures for segregating MLCs into blastocyst cell fates and how to create embryo-like structures from them. This system provides a stem-cell-based embryo model to study early embryo development. For complete details on the use and execution of this protocol, please refer to Li et al..

摘要

生成具有完全发育潜能的胚胎细胞特性的细胞类型具有重要的生物学意义。本文提供了一种生成类似于 8-16 细胞期胚胎细胞的小鼠桑椹胚样细胞（MLC）的方案。我们描述了通过增加 Stat3 激活来诱导 MLC 形成的步骤，以及 MLC 的分离方法。然后详细介绍了将 MLC 分离为囊胚细胞命运的程序，以及如何从它们中创建类胚胎结构。该系统提供了基于干细胞的胚胎模型，可用于研究早期胚胎发育。有关该方案使用和实施的完整详细信息，请参阅 Li 等人的文章。