Durable bonding to zirconia is a challenging issue in dentistry. This study aimed to assess the effect of bioglass coating of zirconia on the microshear bond strength of resin cement to zirconia and to study the effect of thermocycling on this bond. This in-vitro experimental study was conducted on 60 yttria-stabilized tetragonal zirconia blocks in six groups (N=10) based on surface pretreatment and thermocycling. Surface pretreatments included no treatment control, alumina particle abrasion, and bioglass-coating of zirconia. Resin bonding was performed with Panavia F2.0 cements. Then, half of the specimens underwent a 24-hour incubation in 37°C water, while the other half were subjected to thermocycling (12000 cycles, 5-55°C, 60s for each batch) following the same incubation period. Subsequently, the microshear bond strength of the specimens was measured. Additionally, one block from each group was subjected to scanning electron microscopy and X-ray diffraction. The data were analyzed using Kruskal-Wallis and Mann-Whitney U tests. There was a significant difference between the bond strength values of different groups (P<0.001). Alumina particle abrasion and bioglass coating equally increased the bond strength compared to the untreated control group (P<0.001). Thermocycling caused significant decreases in bond strength in all the groups (P<0.001); however, the bond strength value of the thermocycled bioglass-coated group was significantly higher than that reported for the thermocycled alumina particle abraded group (P=0.015). Despite the decrease in the bond strength values after thermocycling, the long-term efficacy of the bioglass coating of zirconia was promising.