Teng Fei, Sun Xiaojing, Ran Yuge, Liu Chan, Shi Hongyun, Tian Yuan
Department of Radiotherapy, Affiliated Hospital of Hebei University, Baoding, China.
Comb Chem High Throughput Screen. 2025;28(3):487-499. doi: 10.2174/0113862073275640231228124547.
Lung adenocarcinoma (LUAD) is a common malignant tumor with no obvious clinical symptoms in its early stages. Patients can be divided into radiotherapysensitive groups (RS) and radiotherapy-resistant groups (RR) due to their varying conditions. The therapeutic effect of radiotherapy is quite different between the two groups. Therefore, this paper explores the role of radiation-related lung function genes in LUAD and its immune landscape.
Firstly, we divided LUAD samples from the TCGA cohort into RS and RR groups and analyzed differential expression to obtain differentially expressed genes (DEGs). Then, DEGs and patients' grouping information were input into the weighted co-expression network, and the genes in the radiotherapy-related modules were identified. Furthermore, after the intersection of DEGs and lung function-related genes, the prognosis-related genes were obtained through univariate Cox and Lasso-Cox analyses, respectively, and the risk model was constructed. Finally, the differences in prognosis and immunity of the samples in the risk model were explored. Additionally, we also performed a qPCR experiment on lung function-related genes.
In this paper, radiation-related genes of LUAD were identified through a series of bioinformatics analyses. By conducting enrichment analysis on these genes, several pathways related to LUAD radiation were identified, and DEGs associated with significant prognosis were determined. Furthermore, a radiation-related risk model of LUAD was developed. All samples were divided into high-risk and low-risk groups based on the risk score, and the differences in immune cell infiltration abundance and immune function between these groups were evaluated. The qPCR experimental results demonstrated a significant difference in the expression of genes related to lung function.
The prognosis-related genes identified in this paper and the risk model created can serve as a reference for diagnosing and treating LUAD.
肺腺癌（LUAD）是一种常见的恶性肿瘤，早期无明显临床症状。由于病情不同，患者可分为放疗敏感组（RS）和放疗抵抗组（RR）。两组放疗的治疗效果差异很大。因此，本文探讨辐射相关肺功能基因在LUAD及其免疫格局中的作用。
首先，我们将来自TCGA队列的LUAD样本分为RS组和RR组，并分析差异表达以获得差异表达基因（DEG）。然后，将DEG和患者分组信息输入加权共表达网络，识别放疗相关模块中的基因。此外，在DEG与肺功能相关基因进行交集后，分别通过单变量Cox分析和Lasso-Cox分析获得预后相关基因，并构建风险模型。最后，探讨风险模型中样本的预后和免疫差异。此外，我们还对肺功能相关基因进行了qPCR实验。
本文通过一系列生物信息学分析鉴定了LUAD的辐射相关基因。通过对这些基因进行富集分析，确定了几个与LUAD辐射相关的途径，并确定了与显著预后相关的DEG。此外，还建立了LUAD的辐射相关风险模型。根据风险评分将所有样本分为高风险组和低风险组，并评估这些组之间免疫细胞浸润丰度和免疫功能的差异。qPCR实验结果表明肺功能相关基因的表达存在显著差异。
本文鉴定的预后相关基因和创建的风险模型可为LUAD的诊断和治疗提供参考。
