Alvarez-Buylla A, Valinsky J E
Proc Natl Acad Sci U S A. 1985 May;82(10):3519-23. doi: 10.1073/pnas.82.10.3519.
Plasminogen activator has been implicated in tissue remodeling and cell migration during embryogenesis. In the developing nervous system, these processes are evident in the migration of neurons, axonal extension, Schwann cell migration, and the ensheathment and myelination of nerves. We have studied the production of plasminogen activator in cultures of superior cervical ganglia under conditions in which both neurons and glia are present. We have found that a principal source of the enzyme in these cultures is the glial cells and that the enzyme could not be detected at the growing tips of neurites. Plasminogen activator is also produced by Schwann cells isolated from neonatal rat sciatic nerve. The production of the enzyme by these cells is stimulated 6- to 10-fold by cholera toxin. Isolated Schwann cells and glial cells in the ganglion explant cultures produce the tissue form of plasminogen activator, a form of the enzyme not often found in nonmalignant cells. Preliminary experiments suggest that neuronal-glial interactions may regulate enzyme production by Schwann cells.
纤溶酶原激活剂与胚胎发育过程中的组织重塑和细胞迁移有关。在发育中的神经系统中，这些过程在神经元迁移、轴突延伸、雪旺细胞迁移以及神经的包被和髓鞘形成中很明显。我们研究了在同时存在神经元和神经胶质细胞的条件下，颈上神经节培养物中纤溶酶原激活剂的产生。我们发现，这些培养物中该酶的主要来源是神经胶质细胞，并且在神经突的生长尖端检测不到该酶。从新生大鼠坐骨神经分离的雪旺细胞也产生纤溶酶原激活剂。霍乱毒素可将这些细胞产生该酶的量刺激6至10倍。神经节外植体培养物中分离的雪旺细胞和神经胶质细胞产生组织型纤溶酶原激活剂，这种酶形式在非恶性细胞中并不常见。初步实验表明，神经元与神经胶质细胞的相互作用可能调节雪旺细胞产生酶的过程。
