Epigenetic Regulation of CYP72A385-Mediated Metabolic Resistance to Novel Auxin Herbicide Florpyrauxifen-benzyl in (L.) P. Beauv.

Weed's metabolic resistance to herbicides has undermined the sustainability of herbicides and global food security. Notably, we identified an (L.) P. Beauv population (R) that evolved resistance to the never-used florpyrauxifen-benzyl, in which florpyrauxifen-benzyl was metabolized faster than the susceptible population (S). RNA-seq identified potential metabolism-related genes, and , whose expression in yeast exhibited the capacity to degrade florpyrauxifen-benzyl. Region-2 in the promoter showed significant demethylation after florpyrauxifen-benzyl treatment in the R population. DNA methyltransferase inhibitors induce overexpression in the S population and endow it with tolerance to florpyrauxifen-benzyl. Moreover, methyltransferase-like 7A () was overexpressed in the S population and specifically bound to the promoter. Transgenic in and L. exhibited resistance to florpyrauxifen-benzyl, whereas transgenic plants were sensitive. Overall, is the principal functional gene for conferring resistance to florpyrauxifen-benzyl and is regulated by in .