[Comparison of the microbiota diversity between autogenous and anautogenous ].

OBJECTIVE

To investigate the microbiota composition and diversity between autogenous and anautogenous , so as to provide insights into unraveling the pathogenesis of autogeny in .

METHODS

Autogenous and anautogenous adult samples were collected at 25 ℃, and the hypervariable regions of the microbial 16S ribosomal RNA () gene was sequenced on the Illumina NovaSeq 6000 sequencing platform. The microbiota abundance and diversity were evaluated using the alpha diversity index, and the difference in the microbiota structure was examined using the beta diversity index. The microbiota with significant differences in the abundance between autogenous and anautogenous adult samples was identified using the linear discriminant analysis effect size (LEfSe).

RESULTS

The microbiota in autogenous and anautogenous samples belonged to 18 phyla, 28 classes, 70 orders, 113 families, and 170 genera, and the dominant phyla included Proteobacteria, Bacteroidetes, and so on. At the genus level, was a common dominant genus, and the relative abundance was (77.6 ± 11.3)% in autogenous samples and (47.5 ± 8.5)% in anautogenous mosquito samples, while (0.4% ± 0.1%), (0.5% ± 0.0%) and (0.5% ± 0.1%) were specific species in autogenous samples. Alpha diversity analysis showed that higher Chao1 index and ACE index in autogenous samples than in anautogenous samples (both values > 0.05), and lower Shannon index ( > 0.05) and Simpson index ( < 0.05) in autogenous samples than in anautogenous samples. LEfSe analysis showed a total of 48 significantly different taxa between autogenous and anautogenous samples (all values < 0.05).

CONCLUSIONS

There is a significant difference in the microbiota diversity between autogenous and anautogenous .