EquiBreed ART Ltd, Te Awamutu, New Zealand.
Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Australia.
Equine Vet J. 2024 Jul;56(4):688-696. doi: 10.1111/evj.14096. Epub 2024 Apr 14.
Insemination of mares with frozen-thawed spermatozoa requires intensive management and results in 40%-60% per cycle pregnancy rates.
To determine if satisfactory fertility is possible for frozen-thawed semen after processing it through a microfluidic device, followed by storage at 17°C for up to 24 h before fixed-time insemination.
Uncontrolled field trials.
A pilot study evaluated the motility of frozen-thawed spermatozoa after centrifugation and storage (17°C) in two different media for up to 48 h. Subsequently, the motility of frozen-thawed semen processed through a microfluidic device, resuspended in two different media during storage (17°C) for up to 24 h was evaluated. The fertility of frozen-thawed spermatozoa, after microfluidic sorting and storage at 17°C for up to 24 h, was evaluated after fixed-time insemination in a commercial embryo programme. Experiment 1: Frozen-thawed spermatozoa (N = 5 stallions) were centrifuged and resuspended in Botusemen Gold™ or SpermSafe™ and stored (17°C) for up to 48 h. Sperm motility was evaluated by CASA at 0, 6, 24 and 48 h. Experiment 2: Frozen-thawed spermatozoa (N = 4 stallions) underwent microfluidic sorting and storage (17°C) for up to 24 h in both media. Sperm concentration and motility were evaluated at 0, 16 and 24 h. Experiment 3: Fertility of frozen-thawed spermatozoa (N = 3 stallions) was evaluated after insemination of 42 mare cycles at 6, 16 and 24 h after thawing, microfluidic sorting and storage before fixed-time insemination.
The stallion significantly influenced sperm motility, but there was no effect of media on motility parameters. Storage time significantly affected sperm motility after centrifugation but not after microfluidic sorting. Storage time had no effect on the overall embryo recovery rate (52%, n = 42).
Field trial with small mare numbers and no control at time = 0 h.
Fixed-time insemination of frozen-thawed spermatozoa after microfluidic sorting and storage at 17°C for up to 24 h produced satisfactory embryo recovery rates.
使用冷冻解冻精子对母马进行授精需要进行密集的管理，每周期妊娠率为 40%-60%。
确定在使用微流控设备处理冷冻解冻精液后，是否可以在 17°C 下储存长达 24 小时，然后再进行定时授精，从而获得满意的生育能力。
非对照现场试验。
一项初步研究评估了在两种不同的介质中离心和储存（17°C）长达 48 小时后，冷冻解冻精子的活力。随后，评估了通过微流控设备处理、在两种不同的介质中储存（17°C）长达 24 小时的冷冻解冻精液的活力。在商业胚胎计划中，评估了在微流控分选和在 17°C 下储存长达 24 小时后，冷冻解冻精子的生育能力。实验 1：将 5 匹种马的冷冻解冻精子（N=5）离心并悬浮在 Botusemen Gold™或 SpermSafe™中，在 17°C 下储存长达 48 小时。通过 CASA 在 0、6、24 和 48 小时评估精子活力。实验 2：将 4 匹种马的冷冻解冻精子（N=4）进行微流控分选，并在两种介质中在 17°C 下储存长达 24 小时。在 0、16 和 24 小时评估精子浓度和活力。实验 3：在解冻后 6、16 和 24 小时，以及微流控分选和储存前进行定时授精，评估 3 匹种马的冷冻解冻精子的生育能力，共对 42 匹母马的周期进行了授精。
种马显著影响精子活力，但精子活力参数不受介质影响。离心后储存时间显著影响精子活力，但微流控分选后不影响。储存时间对整体胚胎回收率（52%，n=42）没有影响。
现场试验中母马数量较少，没有 0 小时的对照。
在 17°C 下储存长达 24 小时，对冷冻解冻精子进行微流控分选后进行定时授精，可以获得满意的胚胎回收率。
