School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.
Anal Chem. 2024 Apr 30;96(17):6652-6658. doi: 10.1021/acs.analchem.3c05970. Epub 2024 Apr 17.
A low-triggering potential and a narrow-potential window are anticipated to decrease the electrochemical interference and cross talk of electrochemiluminescence (ECL). Herein, by exploiting the low oxidative potential (0.82 V vs Ag/AgCl) of dihydrolipoic acid-capped sliver nanoclusters (DHLA-AgNCs), a coreactant ECL system of DHLA-AgNCs/hydrazine (NH) is proposed to achieve efficient and oxidative-reduction ECL with a low-triggering potential of 0.82 V (vs Ag/AgCl) and a narrow-potential window of 0.22 V. The low-triggering-potential and narrow-potential-window nature of ECL can be primarily preserved upon labeling DHLA-AgNCs to probe DNA and immobilizing DHLA-AgNCs onto the Au surface via sandwiched hybridization, which eventually enables a selective ECL strategy for the gene assay at +0.82 V. This gene assay strategy can sensitively determine the gene of human papillomavirus from 10 to 1000 pM with a low limit of detection of 5 pM (S/N = 3) and would open a way to improve the applied ECL bioassay.
低触发电位和窄电位窗口有望降低电化学干扰和电化学发光（ECL）的串扰。在此，通过利用二氢硫辛酸封端的银纳米簇（DHLA-AgNCs）的低氧化电位（0.82 V 相对于 Ag/AgCl），提出了 DHLA-AgNCs/肼（NH）的共反应物 ECL 体系，以实现高效和氧化还原 ECL，其触发电位低至 0.82 V（相对于 Ag/AgCl），电位窗口窄至 0.22 V。ECL 的低触发电位和窄电位窗口性质在通过夹心杂交将 DHLA-AgNCs 标记为探针 DNA 并将 DHLA-AgNCs 固定在 Au 表面上之后主要得以保留，这最终使得在 +0.82 V 时能够实现用于基因分析的选择性 ECL 策略。该基因分析策略能够从 10 到 1000 pM 的范围内灵敏地测定人乳头瘤病毒基因，检测限低至 5 pM（S/N = 3），为改进应用于 ECL 生物分析的方法开辟了道路。
