Dräger U C
Proc Natl Acad Sci U S A. 1985 Oct;82(19):6716-20. doi: 10.1073/pnas.82.19.6716.
The localization of calcium binding sites in eyes was determined autoradiographically after extracting endogenous Ca from tissue sections and replacing it with 45Ca. The strongest labeling was associated with pigmented tissues due to the high concentration of melanin, which was shown to bind Ca effectively and in a pH-dependent fashion. The second strongest binding was over the tapetum lucidum of the cat eye, and moderate labeling was associated with eye muscles and epithelium and endothelium of the cornea. The neural retina was generally more lightly labeled than the surrounding tissue of the eye; here the plexiform layers stood out in comparison to the nuclear layers, as did a band located internal to the photoreceptor outer segments. The possibility that the Ca buffering capacity of melanin may represent the common denominator for the various neurological defects found in hypopigmentation mutants is discussed.
在用45Ca取代组织切片中的内源性钙后，通过放射自显影法确定了眼睛中钙结合位点的定位。由于黑色素浓度高，最强的标记与色素组织相关，黑色素能以pH依赖的方式有效结合钙。第二强的结合出现在猫眼的明毯上，中等强度的标记与眼肌以及角膜的上皮和内皮有关。神经视网膜的标记通常比眼睛周围组织更淡；在这里，与核层相比，神经纤维层很突出，感光细胞外段内部的一条带也是如此。文中讨论了黑色素的钙缓冲能力可能是色素减退突变体中发现的各种神经缺陷的共同特征这一可能性。
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