四川省自贡市慢性乙型肝炎病毒感染者中 MTHFR 和 MTRR 基因多态性。

MTHFR and MTRR gene polymorphisms in patients with chronic hepatitis B virus infections in Zigong, Sichuan Province.

机构信息

Department of Clinical Laboratory, Zigong Third People's Hospital, Zigong City, Sichuan Province, P.R. China.

Department of Clinical Pharmacy, Zigong Third People's Hospital, Zigong City, Sichuan Province, P.R. China.

出版信息

Ann Hum Biol. 2024 Feb;51(1):2330926. doi: 10.1080/03014460.2024.2330926. Epub 2024 Apr 18.

DOI:10.1080/03014460.2024.2330926

PMID:38634541

Abstract

BACKGROUND

Chronic hepatitis B virus (HBV) infection is a severe disease affecting the physical and economic well-being of patients. The relationship between polymorphisms in the MTHFR gene and disease progression following HBV infection remains a controversial topic.

AIM

To study MTHFR and MTRR gene polymorphisms in patients with chronic HBV infections in Zigong, Sichuan Province.

SUBJECTS AND METHODS

One hundred and ninety-one patients with chronic HBV infections were divided into three groups: the chronic hepatitis B (CHB) group ( = 71), the hepatitis B-induced liver cirrhosis (LC) group ( = 56), and the hepatitis B-related primary liver cancer (PLC) group ( = 64). The gene polymorphisms were detected using the PCR-melt curve method and analysed.

RESULTS

The distributions of MTHFR C677T (CC: 41.2% vs. 41.8%; CT: 50% vs. 45.5%; TT: 8.8% vs. 12.7%; = 0.714), MTHFR A1298C (AA: 70.6% vs. 72.7%; AC: 26.5% vs. 25.5%; CC: 2.9% vs. 1.8%; = 1.000), and MTRR A66G (AA: 58.1% vs. 65.5%; AG: 39.0% vs. 29.1%; 2.9% vs. 5.5%; = 0.353) genetic polymorphisms did not vary between male and female patients from Zigong. In addition, there were no differences in the distributions of MTHFR C677T (CC: 43.4% vs. 38.8%; CT: 49.1% vs. 48.2%; TT: 7.5% vs. 12.9%; = 0.444), MTHFR A1298C (AA: 76.4% vs. 64.7%; AC: 20.8% vs. 32.9%; CC: 2.8% vs. 2.4%; = 0.155), and MTRR A66G (AA: 62.3% vs. 57.6%; AG: 34.0% vs. 38.8%; 3.8% vs. 3.5%; = 0.353) genetic polymorphisms between the patients <60 and >60 years of age. The distributions of MTHFR C677T (CHB vs. LC, = 0.888; CHB vs. PLC, = 0.661; PLC vs. LC, = 0.926), MTHFR A1298C (CHB vs. LC, = 0.12; CHB vs. PLC, = 0.263; PLC vs. LC, = 0.550), and MTRR A66G (CHB vs. LC, = 0.955; CHB vs. PLC, = 0.645; PLC vs. LC, = 0.355) gene polymorphisms were comparable between the CHB, LC, and PLC groups.

CONCLUSION

The distributions of MTHFR and MRRR genetic polymorphisms in the population with HBV infections in Zigong, Sichuan Province did not differ in age and sex. The MTHFR and MRRR genetic polymorphisms were comparable between the CHB, LC, and PLC groups.

摘要

背景

慢性乙型肝炎病毒（HBV）感染是一种严重的疾病，会影响患者的身心健康和经济状况。MTHFR 基因多态性与 HBV 感染后疾病进展之间的关系仍然存在争议。

目的

研究四川省自贡市慢性 HBV 感染者 MTHFR 和 MTRR 基因多态性。

受试者和方法

将 191 例慢性 HBV 感染者分为三组：慢性乙型肝炎（CHB）组（n=71）、乙型肝炎相关性肝硬化（LC）组（n=56）和乙型肝炎相关性原发性肝癌（PLC）组（n=64）。采用 PCR-熔解曲线法检测基因多态性并进行分析。

结果

MTHFR C677T（CC：41.2% vs. 41.8%；CT：50% vs. 45.5%；TT：8.8% vs. 12.7%； = 0.714）、MTHFR A1298C（AA：70.6% vs. 72.7%；AC：26.5% vs. 25.5%；CC：2.9% vs. 1.8%； = 1.000）和 MTRR A66G（AA：58.1% vs. 65.5%；AG：39.0% vs. 29.1%； 2.9% vs. 5.5%； = 0.353）的基因多态性在自贡市男性和女性患者之间没有差异。此外，MTHFR C677T（CC：43.4% vs. 38.8%；CT：49.1% vs. 48.2%；TT：7.5% vs. 12.9%； = 0.444）、MTHFR A1298C（AA：76.4% vs. 64.7%；AC：20.8% vs. 32.9%；CC：2.8% vs. 2.4%； = 0.155）和 MTRR A66G（AA：62.3% vs. 57.6%；AG：34.0% vs. 38.8%； 3.8% vs. 3.5%； = 0.353）的基因多态性在年龄<60 岁和>60 岁的患者之间也没有差异。MTHFR C677T（CHB vs. LC， = 0.888；CHB vs. PLC， = 0.661；PLC vs. LC， = 0.926）、MTHFR A1298C（CHB vs. LC， = 0.12；CHB vs. PLC， = 0.263；PLC vs. LC， = 0.550）和 MTRR A66G（CHB vs. LC， = 0.955；CHB vs. PLC， = 0.645；PLC vs. LC， = 0.355）的基因多态性在 CHB、LC 和 PLC 组之间无差异。