School of Life Sciences, Jiangsu University, Zhenjiang, 212013, China.
School of Life Sciences, Jiangsu University, Zhenjiang, 212013, China.
Dev Comp Immunol. 2024 Jul;156:105183. doi: 10.1016/j.dci.2024.105183. Epub 2024 Apr 16.
Bombyx mori nucleopolyhedrovirus (BmNPV) is the most important virus that threatens sericulture industry. At present, there is no effective treatment for BmNPV infection in silkworms, and lncRNA plays an important role in biological immune response and host-virus interaction, but there are relatively few studies in silkworms. In this study, the four midgut tissue samples of the resistance strain NB (NB) and susceptible strain 306 (306) and the NB and 306 continuously infected with BmNPV for 96 h are used for whole transcriptome sequencing to analyze the differences in the genetic background of NB and 306 and the differences after inoculation of BmNPV, and the significantly different mRNA, miRNA and lnRNA between NB and 306 after BmNPV inoculation were screened. By comparing NB and 306, 2651 significantly different mRNAs, 57 significantly different miRNAs and 198 significantly different lncRNAs were screened. By comparing NB and 306 after BmNPV inoculation, 2684 significantly different mRNAs, 39 significantly different miRNAs and 125 significantly different lncRNAs were screened. According to the significantly different mRNA, miRNA and lncRNA screened from NB and 306 and NB and 306 after virus inoculation, the mRNA-miRNA-lncRNA regulatory network was constructed before and after virus inoculation, and the BmBCAT-Bomo_chr7_8305-MSTRG.3236.2 regulatory axis was screened from them, and it was found that BmBCAT was not Bomo_chr7_8305 regulated in the genetic background, after viral infection, MSTRG.3236.2 competes for binding Bomo_chr7_8305 regulates BmBCAT. The whole transcriptome sequencing results were verified by qPCR and the time-series expression analysis was performed to prove the reliability of the regulatory network. The BmBCAT-Bomo_chr7_8305-MSTRG.3236.2 regulatory axis may play a potential role in the interaction between silkworms and BmNPV. These results provide new insights into the interaction mechanism between silkworms and BmNPV.
家蚕核型多角体病毒（BmNPV）是威胁蚕桑业的最重要病毒。目前，家蚕感染 BmNPV 尚无有效治疗方法，而长非编码 RNA 在生物免疫反应和宿主-病毒相互作用中发挥重要作用，但在家蚕中研究相对较少。本研究以抗性品系 NB（NB）和敏感品系 306（306）的 4 个中肠组织样本和连续感染 BmNPV96 h 的 NB 和 306 为材料进行全转录组测序，分析 NB 和 306 的遗传背景差异以及接种 BmNPV 后的差异，筛选接种 BmNPV 后 NB 和 306 之间差异表达的 mRNA、miRNA 和 lncRNA。通过比较 NB 和 306，筛选出 2651 个差异表达 mRNA、57 个差异表达 miRNA 和 198 个差异表达 lncRNA。通过比较接种 BmNPV 前后的 NB 和 306，筛选出 2684 个差异表达 mRNA、39 个差异表达 miRNA 和 125 个差异表达 lncRNA。根据 NB 和 306 以及接种病毒前后 NB 和 306 筛选出的差异表达 mRNA、miRNA 和 lncRNA，构建病毒接种前后的 mRNA-miRNA-lncRNA 调控网络，从中筛选出 BmBCAT-Bomo_chr7_8305-MSTRG.3236.2 调控轴，并发现 BmBCAT 在遗传背景中不受 Bomo_chr7_8305 调控，病毒感染后，MSTRG.3236.2 竞争结合 Bomo_chr7_8305 调控 BmBCAT。通过 qPCR 对全转录组测序结果进行验证，并进行时间序列表达分析，证明调控网络的可靠性。BmBCAT-Bomo_chr7_8305-MSTRG.3236.2 调控轴可能在家蚕与 BmNPV 相互作用中发挥潜在作用。这些结果为家蚕与 BmNPV 相互作用的机制提供了新的见解。
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