National Key Laboratory for Conservation and Utilization of Bio-Resources, Kunming 650201, China.
College of Plant Protection, Yunnan Agricultural University, Kunming 650201,China.
Ying Yong Sheng Tai Xue Bao. 2024 Mar 18;35(3):858-866. doi: 10.13287/j.1001-9332.202403.032.
Insect visual electrophysiological techniques are important to study the electrical characteristics of photoreceptor cells and visual neurons in insects, including electroretinography (ERG) and microelectrode intracellular recording (MIR). ERG records the changes of voltage or electric current in the retina of insects in response to different light stimuli, which occurs outside the cell. MIR records the changes in individual photoreceptor cells or visual neurons of an insect exposed to different lights, which occurs inside the cell. Insect visual electrophysiological techniques can explore the mechanism of electrophysiological response of insects' vision to light and reveal their sensitive light spectra and photoreceptor types. This review introduced the basic structure and the principle of ERG and MIR, and summarized their applications in insect researches in the past 20 years, which would provide references for elucidating the mechanism of light perception in insects and the use of insect phototropism to control pests.
昆虫视觉电生理学技术对于研究昆虫光感受器细胞和视觉神经元的电特性非常重要,包括视网膜电图(ERG)和微电极细胞内记录(MIR)。ERG 记录昆虫视网膜对不同光刺激的电压或电流变化,这种变化发生在细胞外。MIR 记录昆虫暴露于不同光线时单个光感受器细胞或视觉神经元的变化,这种变化发生在细胞内。昆虫视觉电生理学技术可以探索昆虫视觉对光的电生理反应的机制,揭示它们的敏感光谱和光感受器类型。本文介绍了 ERG 和 MIR 的基本结构和原理,并总结了它们在过去 20 年昆虫研究中的应用,这将为阐明昆虫的光感知机制以及利用昆虫趋光性来控制害虫提供参考。