Durbin J E, Fallon A M
Gene. 1985;36(1-2):173-8. doi: 10.1016/0378-1119(85)90082-4.
A recombinant plasmid in which the bacterial chloramphenicol acetyltransferase (CAT) gene is under the control of the Drosophila heat-shock protein (hsp) 70 promoter has been introduced into cultured mosquito (Aedes albopictus) cells using 1,5-dimethyl-1,5-diazaundecamethylene polymethobromide (polybrene) and dimethylsulfoxide (DMSO). CAT activity was induced by incubating transfected cells at 37 degrees C, and high levels of enzyme activity were maintained for more than 24 h after the temperature shock. Transfected DNA was maintained in the cells for at least 4 days. These experiments document an effective method for introducing purified DNA into cultured mosquito cells.
一种重组质粒已被导入培养的蚊子（白纹伊蚊）细胞中，该质粒中细菌氯霉素乙酰转移酶（CAT）基因受果蝇热休克蛋白（hsp）70启动子的控制，使用的方法是1,5 - 二甲基 - 1,5 - 二氮杂十一亚甲基聚甲溴化物（聚凝胺）和二甲基亚砜（DMSO）。通过在37℃孵育转染细胞来诱导CAT活性，温度休克后高水平的酶活性维持超过24小时。转染的DNA在细胞中至少维持4天。这些实验证明了一种将纯化DNA导入培养的蚊子细胞的有效方法。
