Volkmar Marianne, Maus Anna-Lena, Weisbrodt Martin, Bohlender Jonathan, Langsdorf Alexander, Holtmann Dirk, Ulber Roland
Institute of Bioprocess Engineering, University of Kaiserslautern-Landau, Gottlieb-Daimler-Straße 49, 67663, Kaiserslautern, Germany.
Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstraße 14, 35390, Giessen, Germany.
Bioresour Bioprocess. 2023 Jul 22;10(1):43. doi: 10.1186/s40643-023-00663-2.
In Germany alone, more than 5·10 tons of municipal green waste is produced each year. So far, this material is not used in an economically worthwhile way. In this work, grass clippings and tree pruning as examples of municipal green waste were utilized as feedstock for the microbial production of platform chemicals. A pretreatment procedure depending on the moisture and lignin content of the biomass was developed. The suitability of grass press juice and enzymatic hydrolysate of lignocellulosic biomass pretreated with an organosolv process as fermentation medium or medium supplement for the cultivation of Saccharomyces cerevisiae, Lactobacillus delbrueckii subsp. lactis, Ustilago maydis, and Clostridium acetobutylicum was demonstrated. Product concentrations of 9.4 g L, 16.9 g L, 20.0 g L, and 15.5 g L were achieved in the different processes. Yields were in the same range as or higher than those of reference processes grown in established standard media. By reducing the waste arising in cities and using municipal green waste as feedstock to produce platform chemicals, this work contributes to the UN sustainability goals and supports the transition toward a circular bioeconomy.
仅在德国，每年就产生超过510吨的城市绿色垃圾。到目前为止，这种材料尚未得到经济上可行的利用。在这项工作中，以草屑和树木修剪废弃物作为城市绿色垃圾的示例，用作微生物生产平台化学品的原料。开发了一种取决于生物质水分和木质素含量的预处理程序。证明了经有机溶剂法预处理的木质纤维素生物质的草压汁和酶解产物作为发酵培养基或培养基补充物用于酿酒酵母、德氏乳杆菌乳酸亚种、玉米黑粉菌和丙酮丁醇梭菌培养的适用性。在不同过程中实现的产物浓度分别为9.4 g/L、16.9 g/L、20.0 g/L和15.5 g/L。产量与在既定标准培养基中生长的参考过程相同或更高。通过减少城市中产生的废物并将城市绿色垃圾用作生产平台化学品的原料，这项工作有助于实现联合国可持续发展目标，并支持向循环生物经济的转型。
