Department of Rheumatology and Immunology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China.
Int J Rheum Dis. 2024 Apr;27(4):e15145. doi: 10.1111/1756-185X.15145.
To determine the alteration of peripheral T and B cell subsets in patients with systemic sclerosis (SSc) and to evaluate their correlation with the progression of SSc.
We recruited 47 SSc patients and 45 healthy controls (HCs) in this study. Demographic and clinical data were then collected. Flow cytometry was used to detect the proportions of 44 different T and B cell subsets in circulating blood.
The proportion of total B cells (p = .043) decreased in SSc patients, together with similar frequencies of total T cells, CD4+ T cells, and CD8+ T cells in both groups. Several subsets of T and B cells differed significantly between these two groups. Follicular helper T cells-1 (Tfh1) (p < .001), helper T cells-1 (Th1) (p = .001), regulatory T cells (Treg) (p = .004), effector memory CD8+ T cells (p = .041), and cytotoxic T cells-17 (Tc17) (p = .01) were decreased in SSc patients. Follicular helper T cells-2 (Tfh2) (p = .001) and, helper T cells-2 (Th2) (p = .001) levels increased in the SSc group. Regulatory B cells (Breg) (p = .015) were lower in the SSc group, together with marginal zone (MZ) B cells (p < .001), memory B cells (p = .001), and non-switched B cells (p = .005). The modified Rodnan skin score (mRSS) correlated with helper T cells-17 (Th17) (r = -.410, p = .004), Tfh1 (r = -.321, p = .028), peripheral helper T cells (Tph) (r = -.364, p = .012) and plasma cells (r = -.312, p = .033).
The alterations in T and B cells implied immune dysfunction, which may play an essential role in systemic sclerosis.
确定系统性硬化症（SSc）患者外周 T 细胞和 B 细胞亚群的改变，并评估其与 SSc 进展的相关性。
本研究纳入了 47 例 SSc 患者和 45 名健康对照者（HCs）。收集人口统计学和临床数据。采用流式细胞术检测循环血中 44 种不同 T 和 B 细胞亚群的比例。
SSc 患者总 B 细胞比例降低（p=0.043），两组总 T 细胞、CD4+T 细胞和 CD8+T 细胞的频率相似。两组间 T 细胞和 B 细胞的多个亚群存在显著差异。滤泡辅助 T 细胞-1（Tfh1）（p<0.001）、辅助 T 细胞-1（Th1）（p=0.001）、调节性 T 细胞（Treg）（p=0.004）、效应记忆 CD8+T 细胞（p=0.041）和细胞毒性 T 细胞-17（Tc17）（p=0.01）在 SSc 患者中减少。滤泡辅助 T 细胞-2（Tfh2）（p=0.001）和辅助 T 细胞-2（Th2）（p=0.001）水平在 SSc 组中升高。调节性 B 细胞（Breg）（p=0.015）和边缘区（MZ）B 细胞（p<0.001）、记忆 B 细胞（p=0.001）和非转换 B 细胞（p=0.005）在 SSc 组中降低。改良 Rodnan 皮肤评分（mRSS）与辅助 T 细胞-17（Th17）（r=-0.410，p=0.004）、Tfh1（r=-0.321，p=0.028）、外周辅助 T 细胞（Tph）（r=-0.364，p=0.012）和浆细胞（r=-0.312，p=0.033）相关。
T 细胞和 B 细胞的改变暗示免疫功能障碍，这可能在系统性硬化症中起重要作用。
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